ProtParam
User-provided sequence:
10 20 30 40 50 60
MGLKGFAEGG IASIVAGCST HPLDLIKVRM QLQGESAPIQ TNLRPALAFQ TSTTVNAPPL
70 80 90 100 110 120
RVGVIGVGSR LIREEGMRAL FSGVSATVLR QTLYSTTRMG LYDIIKGEWT DPETKTMPLM
130 140 150 160 170 180
KKIGAGAIAG AIGAAVGNPA DVAMVRMQAD GRLPLTDRRN YKSVLDAITQ MIRGEGVTSL
190 200 210 220 230 240
WRGSSLTINR AMLVTSSQLA SYDSVKETIL EKGLLKDGLG THVSASFAAG FVASVASNPV
250 260 270 280 290 300
DVIKTRVMNM KVVAGVAPPY KGAVDCALKT VKAEGIMSLY KGFIPTVSRQ APFTVVLFVT
310
LEQVKKLFKD YDF
References and
documentation are available.
Number of amino acids: 313
Molecular weight: 33357.01
Theoretical pI: 9.74
Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 36
Atomic composition:
Carbon C 1484
Hydrogen H 2434
Nitrogen N 406
Oxygen O 432
Sulfur S 15
Formula: C
1484H
2434N
406O
432S
15
Total number of atoms: 4771
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 21555
Abs 0.1% (=1 g/l) 0.646, assuming all pairs of Cys residues form cystines
Ext. coefficient 21430
Abs 0.1% (=1 g/l) 0.642, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 21.63
This classifies the protein as stable.
Aliphatic index: 97.51
Grand average of hydropathicity (GRAVY): 0.181