ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGLNFREKAW ILLGILCCSS LICSVKAIVT YDRKAVIING QRRILLSGSI HYPRSTPEMW 

        70         80         90        100        110        120 
PDLIQKAKDG GLDVIQTYVF WNGHEPSPGQ YYFEDRYDLV KFIKVVQQAG LYVHLRIGPY 

       130        140        150        160        170        180 
VCAEWNFGGF PVWLKYVPGM VFRTDNEPFK AAMQKFTEKI VRMMKEEKLF ETQGGPIILS 

       190        200        210        220        230        240 
QIENEYGPIE WEIGAPGKAY TKWVAEMAQG LSTGVPWIMC KQDDAPNSII NTCNGFYCEN 

       250        260        270        280        290        300 
FKPNSDNKPK MWTENWTGWF TEFGGAVPYR PAEDIALSVA RFIQNGGSFI NYYMYHGGTN 

       310        320        330        340        350        360 
FDRTAGEFIA TSYDYDAPLD EYGLPREPKY SHLKRLHKVI KLCEPALVSA DPTVTSLGDK 

       370        380        390        400        410        420 
QEAHVFKSKS SCAAFLSNYN TSSAARVLFG GSTYDLPPWS VSILPDCKTE YYNTAKVQVR 

       430        440        450        460        470        480 
TSSIHMKMVP TNTPFSWGSY NEEIPSANDN GTFSQDGLVE QISITRDKTD YFWYLTDITI 

       490        500        510        520        530        540 
SPDEKFLTGE DPLLTIGSAG HALHVFVNGQ LAGTAYGSLE KPKLTFSQKI KLHAGVNKLA 

       550        560        570        580        590        600 
LLSTAAGLPN VGVHYETWNT GVLGPVTLNG VNSGTWDMTK WKWSYKIGTK GEALSVHTLA 

       610        620        630        640        650        660 
GSSTVEWKEG SLVAKKQPLT WYKSTFDSPT GNEPLALDMN TMGKGQMWIN GQNIGRHWPA 

       670        680        690        700        710        720 
YTARGKCERC SYAGTFTEKK CLSNCGEASQ RWYHVPRSWL KPTNNLVIVL EEWGGEPNGI 


SLVKRTAK 

References and documentation are available.

Number of amino acids: 728

Molecular weight: 81532.84

Theoretical pI: 8.03

Amino acid composition: 
Ala (A)  46	  6.3%
Arg (R)  23	  3.2%
Asn (N)  37	  5.1%
Asp (D)  29	  4.0%
Cys (C)  14	  1.9%
Gln (Q)  22	  3.0%
Glu (E)  42	  5.8%
Gly (G)  64	  8.8%
His (H)  15	  2.1%
Ile (I)  40	  5.5%
Leu (L)  55	  7.6%
Lys (K)  51	  7.0%
Met (M)  16	  2.2%
Phe (F)  29	  4.0%
Pro (P)  41	  5.6%
Ser (S)  51	  7.0%
Thr (T)  51	  7.0%
Trp (W)  25	  3.4%
Tyr (Y)  34	  4.7%
Val (V)  43	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 71
Total number of positively charged residues (Arg + Lys): 74

Atomic composition:

Carbon      C	      3695
Hydrogen    H	      5616
Nitrogen    N	       962
Oxygen      O	      1066
Sulfur      S	        30

Formula: C3695H5616N962O1066S30
Total number of atoms: 11369

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   189035
Abs 0.1% (=1 g/l)   2.319, assuming all pairs of Cys residues form cystines


Ext. coefficient   188160
Abs 0.1% (=1 g/l)   2.308, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.75
This classifies the protein as stable.



Aliphatic index: 74.34

Grand average of hydropathicity (GRAVY): -0.331