ProtParam
User-provided sequence:
10 20 30 40 50 60
MGLNFTKLFS RLFAKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN
70 80 90 100 110 120
ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRDRV VEARDELHRM LNEDELRDAV
130 140 150 160 170 180
LLVFANKQDL PNAMNAAEIT DKLGLHSLRQ RHWYIQSTCA TSGEGLYEGL DWLSNNIATK
VNNLNIPF
References and
documentation are available.
Number of amino acids: 188
Molecular weight: 21534.73
Theoretical pI: 6.43
Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 22
Atomic composition:
Carbon C 968
Hydrogen H 1526
Nitrogen N 264
Oxygen O 280
Sulfur S 6
Formula: C
968H
1526N
264O
280S
6
Total number of atoms: 3044
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 29450
Abs 0.1% (=1 g/l) 1.368, assuming all pairs of Cys residues form cystines
Ext. coefficient 29450
Abs 0.1% (=1 g/l) 1.368, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.50
This classifies the protein as stable.
Aliphatic index: 98.51
Grand average of hydropathicity (GRAVY): -0.206