ProtParam
User-provided sequence:
10 20 30 40 50 60
MGLSFGKLFS KLFAKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN
70 80 90 100 110 120
ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRDRV VEARDELHRM LNEDELRDAV
130 140 150 160 170 180
LLVFANKQDL PNAMNAAEIT DKLGLHSLRQ RHWYIQSTCA TSGEGLYEGL DWLSNNIANK
A
References and
documentation are available.
Number of amino acids: 181
Molecular weight: 20607.65
Theoretical pI: 6.43
Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 22
Atomic composition:
Carbon C 925
Hydrogen H 1460
Nitrogen N 252
Oxygen O 269
Sulfur S 6
Formula: C
925H
1460N
252O
269S
6
Total number of atoms: 2912
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 29450
Abs 0.1% (=1 g/l) 1.429, assuming all pairs of Cys residues form cystines
Ext. coefficient 29450
Abs 0.1% (=1 g/l) 1.429, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 30.64
This classifies the protein as stable.
Aliphatic index: 96.96
Grand average of hydropathicity (GRAVY): -0.217