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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGLSSIFGGG APSQQKEAAT TAKTTPNPIA KELKNQIAQE LAVANATELV NKISENCFEK

70 80 90 100
CLTSPYATRN DACIDQCLAK YMRSWNVISK AYISRIQNAS ASGEI


References and documentation are available.

Number of amino acids: 105 Molecular weight: 11285.82 Theoretical pI: 8.38
Amino acid composition: 
Ala (A) 15 14.3% Arg (R) 3 2.9% Asn (N) 8 7.6% Asp (D) 2 1.9% Cys (C) 4 3.8% Gln (Q) 6 5.7% Glu (E) 7 6.7% Gly (G) 5 4.8% His (H) 0 0.0% Ile (I) 9 8.6% Leu (L) 6 5.7% Lys (K) 8 7.6% Met (M) 2 1.9% Phe (F) 2 1.9% Pro (P) 4 3.8% Ser (S) 10 9.5% Thr (T) 7 6.7% Trp (W) 1 1.0% Tyr (Y) 3 2.9% Val (V) 3 2.9% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 9 Total number of positively charged residues (Arg + Lys): 11 Atomic composition: Carbon C 487 Hydrogen H 791 Nitrogen N 137 Oxygen O 158 Sulfur S 6 Formula: C487H791N137O158S6 Total number of atoms: 1579 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 10220 Abs 0.1% (=1 g/l) 0.906, assuming all pairs of Cys residues form cystines Ext. coefficient 9970 Abs 0.1% (=1 g/l) 0.883, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 52.02 This classifies the protein as unstable. Aliphatic index: 78.29 Grand average of hydropathicity (GRAVY): -0.276