ProtParam
User-provided sequence:
10 20 30 40 50 60
MGLVDGYDTS SDSDLNFDEG KSVHEKKNGN LHEDTSYEPS SNNIHKRKSH FTKSELKRRR
70 80 90 100 110 120
KTRKGDGPWG SWSSSDDETS QASETQKEDQ DIFVHALAED NLDSEQIEVE EVSHFYGKSE
130 140 150 160 170 180
KDYQGRGYLY PPNDVDVDLR EERISFRCYL PKKVIRNYPG HPEGTTALKF LPKTGHLILS
190 200 210 220 230 240
GGNDHTIKIW DFYHDYECLR DFQGHNKPIK ALRFTEDCQS FLSSSFDRSV KIWDTETGKV
250 260 270 280 290 300
KTRLHLNSTP ADVESRPTNP HEFIVGLSNS KILHYDDRVS ENQGLVQTYD HHLSSILALK
310 320 330 340 350 360
YFPDGSKFIS SSEDKTVRIW ENQINVPIKQ ISDTAQHSMP FLNVHPSQNY FCAQSMDNRI
370 380 390 400 410 420
YSFSLKPKYK RHPKKIFKGH SSAGYGISLA FSGDGRYICS GDSKSRLFTW DWNTSRLLNN
430 440 450
IKIPGNKPIT QVDWHPQETS KVICSGAAGK IYVCD
References and
documentation are available.
Number of amino acids: 455
Molecular weight: 52047.81
Theoretical pI: 6.73
Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 60
Atomic composition:
Carbon C 2303
Hydrogen H 3522
Nitrogen N 648
Oxygen O 715
Sulfur S 10
Formula: C
2303H
3522N
648O
715S
10
Total number of atoms: 7198
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 72685
Abs 0.1% (=1 g/l) 1.397, assuming all pairs of Cys residues form cystines
Ext. coefficient 72310
Abs 0.1% (=1 g/l) 1.389, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.05
This classifies the protein as unstable.
Aliphatic index: 64.46
Grand average of hydropathicity (GRAVY): -0.844