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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGLYASKLFS NLFGNKEMRI LMVGLDGAGK TTVLYKLKLG EVITTIPTIG FNVETVQYKN

70 80 90 100 110 120
ISFTVWDVGG QDRIRSLWRH YYRNTEGVIF VIDSNDRSRI GEAREVMQRM LNEDELRNAV

130 140 150 160 170 180
WLVFANKQDL PEAMSAAEIT EKLGLHSIRN RPWFIQSTCA TSGEGLYEGL EWLSNNLKNQ


S


References and documentation are available.

Number of amino acids: 181 Molecular weight: 20657.60 Theoretical pI: 6.75
Amino acid composition: 
Ala (A) 9 5.0% Arg (R) 12 6.6% Asn (N) 13 7.2% Asp (D) 7 3.9% Cys (C) 1 0.6% Gln (Q) 6 3.3% Glu (E) 14 7.7% Gly (G) 15 8.3% His (H) 2 1.1% Ile (I) 12 6.6% Leu (L) 19 10.5% Lys (K) 9 5.0% Met (M) 6 3.3% Phe (F) 7 3.9% Pro (P) 3 1.7% Ser (S) 12 6.6% Thr (T) 11 6.1% Trp (W) 5 2.8% Tyr (Y) 6 3.3% Val (V) 12 6.6% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 21 Total number of positively charged residues (Arg + Lys): 21 Atomic composition: Carbon C 921 Hydrogen H 1450 Nitrogen N 254 Oxygen O 272 Sulfur S 7 Formula: C921H1450N254O272S7 Total number of atoms: 2904 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 36440 Abs 0.1% (=1 g/l) 1.764, assuming all pairs of Cys residues form cystines Ext. coefficient 36440 Abs 0.1% (=1 g/l) 1.764, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 41.17 This classifies the protein as unstable. Aliphatic index: 90.99 Grand average of hydropathicity (GRAVY): -0.274