ProtParam
User-provided sequence:
10 20 30 40 50 60
MGLYASKLFS NLFGNKEMRI LMVGLDGAGK TTVLYKLKLG EVITTIPTIG FNVETVQYKN
70 80 90 100 110 120
ISFTVWDVGG QDRIRSLWRH YYRNTEGVIF VIDSNDRSRI GEAREVMQRM LNEDELRNAV
130 140 150 160 170 180
WLVFANKQDL PEAMSAAEIT EKLGLHSIRN RPWFIQSTCA TSGEGLYEGL EWLSNNLKNQ
S
References and
documentation are available.
Number of amino acids: 181
Molecular weight: 20657.60
Theoretical pI: 6.75
Total number of negatively charged residues (Asp + Glu): 21
Total number of positively charged residues (Arg + Lys): 21
Atomic composition:
Carbon C 921
Hydrogen H 1450
Nitrogen N 254
Oxygen O 272
Sulfur S 7
Formula: C
921H
1450N
254O
272S
7
Total number of atoms: 2904
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 36440
Abs 0.1% (=1 g/l) 1.764, assuming all pairs of Cys residues form cystines
Ext. coefficient 36440
Abs 0.1% (=1 g/l) 1.764, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.17
This classifies the protein as unstable.
Aliphatic index: 90.99
Grand average of hydropathicity (GRAVY): -0.274