ProtParam
User-provided sequence:
10 20 30 40 50 60
MGNEYYYLFK IVLIGNPGVG KSNLLSRFTR NEFNLKSKPT IGVEFATKII SVEGKAVKVQ
70 80 90 100 110 120
IWDTAGMERF RCGASSYYRG ALGALLVYDI SKHKTYESVE QWLKVLRDHA NEDIVITLVG
130 140 150 160 170 180
NKSDLSHAVP TDEAKIYAER NHISFIETSA LDNTNVEAAF TNIVTEIYKL VSEKYKDHSG
190
TIIPSTASNT PKNQCCFP
References and
documentation are available.
Number of amino acids: 198
Molecular weight: 22150.21
Theoretical pI: 7.70
Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 23
Atomic composition:
Carbon C 995
Hydrogen H 1559
Nitrogen N 263
Oxygen O 299
Sulfur S 5
Formula: C
995H
1559N
263O
299S
5
Total number of atoms: 3121
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 26025
Abs 0.1% (=1 g/l) 1.175, assuming all pairs of Cys residues form cystines
Ext. coefficient 25900
Abs 0.1% (=1 g/l) 1.169, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 19.42
This classifies the protein as stable.
Aliphatic index: 88.13
Grand average of hydropathicity (GRAVY): -0.240