ProtParam
User-provided sequence:
10 20 30 40 50 60
MGNFIITERK KAKEERSNPQ TDSMDDLLIR RLTDRNDKEA HLNELFQDNS GAIGGNIVSH
70 80 90 100 110 120
DDALLNTLAI LQKELKSKEQ EIRRLKEVIA LKNKNTERLN DELISGTIEN NVLQQKLSDL
130 140 150
KKEHSQLVAR WLKKTEKETE AMNSEIDGTK
References and
documentation are available.
Number of amino acids: 150
Molecular weight: 17222.45
Theoretical pI: 5.99
Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 26
Atomic composition:
Carbon C 738
Hydrogen H 1237
Nitrogen N 221
Oxygen O 245
Sulfur S 3
Formula: C
738H
1237N
221O
245S
3
Total number of atoms: 2444
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 0.319
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.75
This classifies the protein as unstable.
Aliphatic index: 91.07
Grand average of hydropathicity (GRAVY): -0.924