ProtParam
User-provided sequence:
10 20 30 40 50 60
MGNFRFPIKT KLPPGFINAR ILRDNFKRQQ FKENEILVKS LKFIARNMNL PTKLRLEAQL
70 80 90 100 110
KLNALPNYMR STQIKNRCVD SGHARFVLSD FRLCRYQFRE NALKGNLPGV KKGIW
References and
documentation are available.
Number of amino acids: 115
Molecular weight: 13538.02
Theoretical pI: 11.04
Total number of negatively charged residues (Asp + Glu): 7
Total number of positively charged residues (Arg + Lys): 24
Atomic composition:
Carbon C 612
Hydrogen H 990
Nitrogen N 182
Oxygen O 155
Sulfur S 5
Formula: C
612H
990N
182O
155S
5
Total number of atoms: 1944
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 8605
Abs 0.1% (=1 g/l) 0.636, assuming all pairs of Cys residues form cystines
Ext. coefficient 8480
Abs 0.1% (=1 g/l) 0.626, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 23.69
This classifies the protein as stable.
Aliphatic index: 86.52
Grand average of hydropathicity (GRAVY): -0.497