ProtParam
User-provided sequence:
10 20 30 40 50 60
MGNIITDTII ITSDKCDIVD NDNVERIQVW LSKNILRKFQ INENEPLQLI ILKRFKRILL
70 80 90 100 110 120
ICPSHDISQH VMDASRALEM ENFNFSYSLQ DGQRNLTKQY LKVPESEKMF LISPPASPPP
130 140 150 160 170 180
EFDFSKCEDA PQRHIQSHIQ QDQQQRLEAS QLLPNNPDKN NNGTFTLLKS KVGAITIDRC
190 200 210
PTNDGNGQMQ LADHVKTAFP PKSIFDTDDD D
References and
documentation are available.
Number of amino acids: 211
Molecular weight: 24135.29
Theoretical pI: 5.22
Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 23
Atomic composition:
Carbon C 1057
Hydrogen H 1680
Nitrogen N 296
Oxygen O 332
Sulfur S 9
Formula: C
1057H
1680N
296O
332S
9
Total number of atoms: 3374
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 8730
Abs 0.1% (=1 g/l) 0.362, assuming all pairs of Cys residues form cystines
Ext. coefficient 8480
Abs 0.1% (=1 g/l) 0.351, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 51.78
This classifies the protein as unstable.
Aliphatic index: 83.65
Grand average of hydropathicity (GRAVY): -0.612