ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGNKKLLTGG SSKTHGSGSS YRDPLLQNQE DKPKANGSEN GLNDLEHGVV EAANVGFGRV 

        70         80         90        100        110        120 
FALAKPDAGK LVIGTIALLI GSTTNLLVPK FGGMIIDIVS RDVKTPEQQT ESLIAVRNAV 

       130        140        150        160        170        180 
VIILLIVVIG SICTALRAWL FNSASERVVA RLRKDLFRHL MHQEIAFYDV TKTGELLSRL 

       190        200        210        220        230        240 
SEDTQIIKNA ATTNLSEALR NVTTALIGVG FMFTSSWKLT LLALVVVPVI SVAVKQFGRY 

       250        260        270        280        290        300 
LRELSHTTQA AAAVAASIAE ESFGAVRTVR SFAKESYMVS QYSKKVDETL KLGLKQAVLV 

       310        320        330        340        350        360 
GLFFGGLNAA FTLSVITVVS YGAYLTIYGS MTVGALTSFI LYSLTVGSSV SSLSSLYTTA 

       370        380        390        400        410        420 
MKAAGASRRV FQILDRVSSM SSSGDKCPVG NPDGDVELND VWFAYPSRPS HMILKGISLR 

       430        440        450        460        470        480 
LTPGSKVALV GPSGGGKTTI ANLIERFYDP LKGKILLNGV SLMEISHQYL HKQISIVSQE 

       490        500        510        520        530        540 
PILFNCSVEE NIAYGFDGEA SFTDIENAAK MANAHEFIEA FPDKYNTVVG ERGLRLSGGQ 

       550        560        570        580        590        600 
KQRIAIARAL LTNPSVLLLD EATSALDAES EYLVQDAMDS LMAGRTVLVI AHRLSTVKTA 

       610        620        630        640 
DCVAVISDGE VAEKGTHDEL LSLNGIYTNL VKRQLQSSSS VTTL 

References and documentation are available.

Number of amino acids: 644

Molecular weight: 69103.51

Theoretical pI: 8.13

Amino acid composition: 
Ala (A)  60	  9.3%
Arg (R)  28	  4.3%
Asn (N)  25	  3.9%
Asp (D)  26	  4.0%
Cys (C)   4	  0.6%
Gln (Q)  19	  3.0%
Glu (E)  33	  5.1%
Gly (G)  51	  7.9%
His (H)  11	  1.7%
Ile (I)  39	  6.1%
Leu (L)  76	 11.8%
Lys (K)  33	  5.1%
Met (M)  13	  2.0%
Phe (F)  23	  3.6%
Pro (P)  16	  2.5%
Ser (S)  65	 10.1%
Thr (T)  43	  6.7%
Trp (W)   3	  0.5%
Tyr (Y)  17	  2.6%
Val (V)  59	  9.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 61

Atomic composition:

Carbon      C	      3080
Hydrogen    H	      4988
Nitrogen    N	       830
Oxygen      O	       932
Sulfur      S	        17

Formula: C3080H4988N830O932S17
Total number of atoms: 9847

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    42080
Abs 0.1% (=1 g/l)   0.609, assuming all pairs of Cys residues form cystines


Ext. coefficient    41830
Abs 0.1% (=1 g/l)   0.605, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.79
This classifies the protein as stable.



Aliphatic index: 105.53

Grand average of hydropathicity (GRAVY): 0.180