ProtParam
User-provided sequence:
10 20 30 40 50 60
MGNNTSRRSQ SQKFKNIPSI SAGSFSTMPV QHRGRRRRSK SIVGRAAQNA VLRSKEKTWI
70 80 90 100 110 120
VPLILLTLLV GWYFVNPNGY IKYGIFLSYP IPGTNPAQYG KGRLDIAFCL FYALFFTFCR
130 140 150 160 170 180
EFIMQEIIAR IGRHFNIRAP AKLRRFEEQA YTCLYFTVMG SWGLYVMKQT PMWFFNTDAF
190 200 210 220 230 240
WEEYPHFYHV GSFKAFYLIE AAYWIQQALV LILQLEKPRK DFKELVVHHI ITLLLIGLSY
250 260 270 280 290 300
YFHFTWIGLA VFITMDTSDI WLALSKCLNY VNTVIVYPIF VIFVFVWIYM RHYLNFKIMW
310 320 330 340 350 360
AVWGTMRTIN SFDLDWAAEQ YKCWISRDVT LILLTALQLV NIYWLILILR IGYRAFTTND
370 380
THDERSEDED EEVSDEKSSA KKND
References and
documentation are available.
Number of amino acids: 384
Molecular weight: 45335.85
Theoretical pI: 9.29
Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 42
Atomic composition:
Carbon C 2123
Hydrogen H 3193
Nitrogen N 533
Oxygen O 542
Sulfur S 15
Formula: C
2123H
3193N
533O
542S
15
Total number of atoms: 6406
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 110030
Abs 0.1% (=1 g/l) 2.427, assuming all pairs of Cys residues form cystines
Ext. coefficient 109780
Abs 0.1% (=1 g/l) 2.421, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.61
This classifies the protein as unstable.
Aliphatic index: 96.74
Grand average of hydropathicity (GRAVY): 0.087