ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGNNYRFKLS ELIPNAWFYK LRDMSKSKKK NLQSQPNSTT SKKKHHAVPT PTSTTPLSPR 

        70         80         90        100        110        120 
PPRRPSHSSK APPSHPPRKS SGNRLRHRAT VDSKSSTTSG DSTTTETGSF SPDFRSDQVL 

       130        140        150        160        170        180 
LPDESLTGSW HSPCSSKLSK TATFTPPPEL ELRPIITKTA ATARKTAVNS PAGVRLRMRS 

       190        200        210        220        230        240 
PRISVSSSAR RSGSSARRSR AVVKASVDPK RDFKESMEEM IAENKIRATK DLEELLACYL 

       250        260        270 
CLNSDEYHAI IINVFKQIWL DLNLPPPHSK 

References and documentation are available.

Number of amino acids: 270

Molecular weight: 30030.13

Theoretical pI: 10.35

Amino acid composition: 
Ala (A)  18	  6.7%
Arg (R)  23	  8.5%
Asn (N)  11	  4.1%
Asp (D)  11	  4.1%
Cys (C)   3	  1.1%
Gln (Q)   4	  1.5%
Glu (E)  12	  4.4%
Gly (G)   7	  2.6%
His (H)   8	  3.0%
Ile (I)  10	  3.7%
Leu (L)  21	  7.8%
Lys (K)  23	  8.5%
Met (M)   5	  1.9%
Phe (F)   7	  2.6%
Pro (P)  26	  9.6%
Ser (S)  42	 15.6%
Thr (T)  22	  8.1%
Trp (W)   3	  1.1%
Tyr (Y)   4	  1.5%
Val (V)  10	  3.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 46

Atomic composition:

Carbon      C	      1306
Hydrogen    H	      2124
Nitrogen    N	       396
Oxygen      O	       400
Sulfur      S	         8

Formula: C1306H2124N396O400S8
Total number of atoms: 4234

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    22585
Abs 0.1% (=1 g/l)   0.752, assuming all pairs of Cys residues form cystines


Ext. coefficient    22460
Abs 0.1% (=1 g/l)   0.748, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 65.04
This classifies the protein as unstable.



Aliphatic index: 62.19

Grand average of hydropathicity (GRAVY): -0.805