ProtParam
User-provided sequence:
10 20 30 40 50 60
MGNQSLVVLT ESKGEYENET ELPVKKSSRD NNIGESLTAT AFTQSEDEMV DSNQKWQNPN
70 80 90 100 110 120
YFKYAWQEYL FIFTCMISQL LNQAGTTQTL SIMNILSDSF GSEGNSKSWL MASFPLVSGS
130 140 150 160 170 180
FILISGRLGD IYGLKKMLLV GYVLVIIWSL ICGITKYSGS DTFFIISRAF QGLGIAFVLP
190 200 210 220 230 240
NVLGIIGNIY VGGTFRKNIV ISFVGAMAPI GATLGCLFAG LIGTEDPKQW PWAFYAYSIA
250 260 270 280 290 300
AFINFVLSIY AIPSTIPTNI HHFSMDWIGS VLGVIGLILL NFVWNQAPIS GWNQAYIIVI
310 320 330 340 350 360
LIISVIFLVV FIIYEIRFAK TPLLPRAVIK DRHMIQIMLA LFFGWGSFGI FTFYYFQFQL
370 380 390 400 410 420
NIRQYTALWA GGTYFMFLIW GIIAALLVGF TIKNVSPSVF LFFSMVAFNV GSIMASVTPV
430 440 450 460 470 480
HETYFRTQLG TMIILSFGMD LSFPASSIIF SDNLPMEYQG MAGSLVNTVV NYSMSLCLGM
490 500 510 520 530 540
GATVETQVNS DGKHLLKGYR GAQYLGIGLA SLACMISGLY MVESFIKGRR ARAAAEYDCT
VA
References and
documentation are available.
Number of amino acids: 542
Molecular weight: 59983.18
Theoretical pI: 6.95
Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 30
Atomic composition:
Carbon C 2791
Hydrogen H 4263
Nitrogen N 665
Oxygen O 749
Sulfur S 27
Formula: C
2791H
4263N
665O
749S
27
Total number of atoms: 8495
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 102135
Abs 0.1% (=1 g/l) 1.703, assuming all pairs of Cys residues form cystines
Ext. coefficient 101760
Abs 0.1% (=1 g/l) 1.696, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.63
This classifies the protein as stable.
Aliphatic index: 106.86
Grand average of hydropathicity (GRAVY): 0.565