ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGNSSSKSSK KDSHSNSSSR NPRPQVSRTE TSHSVKSAKS NKSSRSRRSL PSSSTTNTNS 

        70         80         90        100        110        120 
NVPDPSTPSK PNLEVNHQRH SSHTNRYHFP SSSHSHSNSQ NELLTTPSSS STKRPSTSRR 

       130        140        150        160        170        180 
SSYNTKAAAD LPPSMIQMEP KSPILKTNNS STHVSKHKSS YSSTYYENAL TDDDNDDKDN 

       190        200        210        220        230        240 
DISHTKRFSR SSNSRPSSIR SGSVSRRKSD VTHEEPNNGS YSSNNQENYL VQALTRSNSH 

       250        260        270        280        290        300 
ASSLHSRKSS FGSDGNTAYS TPLNSPGLSK LTDHSGEYFT SNSTSSLNHH SSRDIYPSKH 

       310        320        330        340        350        360 
ISNDDDIENS SQLSNIHASM ENVNDKNNNI TDSKKDPNEE FNDIMQSSGN KNAPKKFKKP 

       370        380        390        400        410        420 
IDIDETIQKL LDAGYAAKRT KNVCLKNNEI LQICIKAREI FLSQPSLLEL SPPVKIVGDV 

       430        440        450        460        470        480 
HGQYGDLLRL FTKCGFPPSS NYLFLGDYVD RGKQSLETIL LLFCYKIKYP ENFFLLRGNH 

       490        500        510        520        530        540 
ECANVTRVYG FYDECKRRCN IKIWKTFIDT FNTLPLAAIV AGKIFCVHGG LSPVLNSMDE 

       550        560        570        580        590        600 
IRHVVRPTDV PDFGLINDLL WSDPTDSPNE WEDNERGVSY CYNKVAINKF LNKFGFDLVC 

       610        620        630        640        650        660 
RAHMVVEDGY EFFNDRSLVT VFSAPNYCGE FDNWGAVMSV SEGLLCSFEL LDPLDSAALK 

       670        680        690 
QVMKKGRQER KLANQQQQMM ETSITNDNES QQ 

References and documentation are available.

Number of amino acids: 692

Molecular weight: 77490.63

Theoretical pI: 8.51

Amino acid composition: 
Ala (A)  25	  3.6%
Arg (R)  35	  5.1%
Asn (N)  63	  9.1%
Asp (D)  44	  6.4%
Cys (C)  12	  1.7%
Gln (Q)  21	  3.0%
Glu (E)  33	  4.8%
Gly (G)  28	  4.0%
His (H)  24	  3.5%
Ile (I)  29	  4.2%
Leu (L)  51	  7.4%
Lys (K)  48	  6.9%
Met (M)  11	  1.6%
Phe (F)  26	  3.8%
Pro (P)  36	  5.2%
Ser (S) 110	 15.9%
Thr (T)  38	  5.5%
Trp (W)   4	  0.6%
Tyr (Y)  22	  3.2%
Val (V)  32	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 77
Total number of positively charged residues (Arg + Lys): 83

Atomic composition:

Carbon      C	      3340
Hydrogen    H	      5239
Nitrogen    N	       981
Oxygen      O	      1101
Sulfur      S	        23

Formula: C3340H5239N981O1101S23
Total number of atoms: 10684

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    55530
Abs 0.1% (=1 g/l)   0.717, assuming all pairs of Cys residues form cystines


Ext. coefficient    54780
Abs 0.1% (=1 g/l)   0.707, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 54.31
This classifies the protein as unstable.



Aliphatic index: 62.11

Grand average of hydropathicity (GRAVY): -0.828