ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGNTDSKSSS ILLNHCIALV RPEDADASSP SRTSSPSPSL SVDADPLSLN LSIFKLDSGP 

        70         80         90        100        110        120 
DVEALFSDKP NVPLDTVFND FYLDFISVDV QDFSINSSFK KILHIISSLN PPNFNNLIVF 

       130        140        150        160        170        180 
LSLYIILSAN SLPASRTGLH SSRLINAIKT LSILIPIYFD RVKSSTQDHY DVFWATQHEI 

       190        200        210        220        230        240 
EGLPLQNIPL GERLLLAILK LAFQDNFTTA VTAHPSELWE IGILTNSNKY RSLLNMHHQW 

       250        260        270        280        290        300 
HLFANRLLLL RLLAALFSSD LYTSGGKQDI NMFLVYWCTQ MPKDKSIQFT SSLLNCTMRF 

       310        320        330        340        350        360 
ILNNNKDFQS LKANFFSSDA TASNWQTLYF QFVQSCLHVL NLSMSYKAQD NVITIFLTQL 

       370        380        390        400        410        420 
QREYDLKLIL SSFIKIFKYP IDLAIEQESN IFNFTNNKHI DASRRRAVST SSHDNSSSSH 

       430        440        450        460        470        480 
ASLPSSSSAA YHTKPQTKPQ LPEIHPLLIP MTILMTNLID CNKCFQNYFA DKFASRFIIF 

       490        500        510        520        530        540 
SIYYLKYYDY SSLSSSSSTT RSNSSTTSNG TSNDTSNERS IVELNENSVS QILLPLLNHL 

       550        560        570        580        590        600 
LLILTSKKLV LFKMLQTFNL NYYTNNLPNF YKLSNINGDI NNLTFRDFTV IQLSNLILDN 

       610        620        630        640        650        660 
IKFNLQPNPI FYELIYNLLP INDEILTSSH KNDDSHDDLI LLSAKKKSAS PSAATSSHTS 

       670        680        690        700        710        720 
SSKLSYNAAM SLLYVLSKSS NKVYLTTYAT PVFKTKDIPY MISPGFKMDL LALLLRSITI 

       730        740        750        760        770        780 
FFTLYFDDAE NLLFAMVRHQ SITHQINDSI NSISKALDMN PNLNSHIMTL KQMGFNRKVQ 

       790        800        810        820        830        840 
WKDFYQFEEI TDLPQVNLYS SANQQHQNQQ QGQNDNRGQN QNEDPGQENE SPTPYLLFNP 

       850        860        870        880        890        900 
ASLENETPGT VKHFSSTNHD KNYQVIAFID FKSDSNLNLQ HQLEYWPHRP QWPTPLTFTH 

       910        920        930        940        950        960 
KCKNPKYENF NEVWSGTVYL QILLRVIKQI LSKVPEIPRI KSVQYFETLS KLSALRSDIL 

       970        980        990       1000       1010       1020 
TTIHPRLPLD VRRLTTFQPL SMHTNDKLLM WFHIATWANI FTQTSFKYEE TFSHELRQFE 

      1030       1040       1050       1060       1070       1080 
SLLDISIDEC EGNTISKPTT DRLGYIRRSR GQSSVSLERT ISAGSGVSTP TMALNRTKSN 

      1090       1100       1110       1120       1130       1140 
GSGNLMNYFF QNTAQNHFQH LRSSSSSSSI TLEKTTSNSS SIRTRPNSHH VAPETNNNNS 

      1150       1160       1170       1180       1190       1200 
TNGNSNNSSN GGFSFFKWKW GGNNSNGGSD DTKASQRDPN VSTSIITDNL NSYMFEEEIS 

      1210       1220       1230       1240       1250       1260 
PGVVNNIIEN NIWVGTDIRL FKIANFRKES FSFLEMTSSF FKKFKFINSD NDNYNNNEFD 

      1270 
DNTQLRYTSR GLYR 

References and documentation are available.

Number of amino acids: 1274

Molecular weight: 145052.45

Theoretical pI: 7.25

Amino acid composition: 
Ala (A)  51	  4.0%
Arg (R)  45	  3.5%
Asn (N) 122	  9.6%
Asp (D)  67	  5.3%
Cys (C)   8	  0.6%
Gln (Q)  56	  4.4%
Glu (E)  45	  3.5%
Gly (G)  34	  2.7%
His (H)  36	  2.8%
Ile (I)  90	  7.1%
Leu (L) 146	 11.5%
Lys (K)  66	  5.2%
Met (M)  22	  1.7%
Phe (F)  78	  6.1%
Pro (P)  55	  4.3%
Ser (S) 167	 13.1%
Thr (T)  87	  6.8%
Trp (W)  14	  1.1%
Tyr (Y)  44	  3.5%
Val (V)  41	  3.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 112
Total number of positively charged residues (Arg + Lys): 111

Atomic composition:

Carbon      C	      6495
Hydrogen    H	     10043
Nitrogen    N	      1739
Oxygen      O	      1975
Sulfur      S	        30

Formula: C6495H10043N1739O1975S30
Total number of atoms: 20282

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   143060
Abs 0.1% (=1 g/l)   0.986, assuming all pairs of Cys residues form cystines


Ext. coefficient   142560
Abs 0.1% (=1 g/l)   0.983, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.85
This classifies the protein as unstable.



Aliphatic index: 85.58

Grand average of hydropathicity (GRAVY): -0.355