ProtParam
User-provided sequence:
10 20 30 40 50 60
MGNTFGSSLA SLFFLWATIQ QIFPNHLRIA IKEFLISTIQ QLSFVQRFSD RFINFFSPYV
70 80 90 100 110 120
EISFSQYEDY QFNHAFAAIE TYLGAKATDK AKHLRASQVK ESKGLVLKRD ETKVRDEYEG
130 140 150 160 170 180
GTVWWEMETD STGYRTFKLT FHRRSRDIVT DSYIKYVFEE GKSIQAKSKQ MKLFTNNPSS
190 200 210 220 230 240
HWGTSKKSFW RYIDFEHPAS FHTLAMDTKK KEEILNDLAA FSNGKEYYKK IGKAWKRGYL
250 260 270 280 290 300
LHGPPGTGKS TMIAAMANHL NYSIYDLELT AIRNNSELRK LLTATSSKSI IVIEDIDCSL
310 320 330 340 350 360
DLTGKRKKEK NLMTSREDGE QGTEEDKSFV TLSGLLNFID GIWSACGQER IIIFTTNHFE
370 380 390 400 410 420
KLDPALIRRG RMDMHIELSY CSFEAFKILA KNYLDLDTHP LFKKIESLLK ETKIAPADVA
430 440 450 460 470
ENLMKKNTEI DADGSLKDLI QALEGKKKIH GAQVDEPKDK YTKKFYKAFC MSSKA
References and
documentation are available.
Number of amino acids: 475
Molecular weight: 54609.45
Theoretical pI: 8.98
Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 71
Atomic composition:
Carbon C 2466
Hydrogen H 3841
Nitrogen N 651
Oxygen O 720
Sulfur S 15
Formula: C
2466H
3841N
651O
720S
15
Total number of atoms: 7693
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 65570
Abs 0.1% (=1 g/l) 1.201, assuming all pairs of Cys residues form cystines
Ext. coefficient 65320
Abs 0.1% (=1 g/l) 1.196, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 30.75
This classifies the protein as stable.
Aliphatic index: 77.49
Grand average of hydropathicity (GRAVY): -0.476