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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGNTFGSSLA SLFFLWATIQ QIFPNHLRIA IKEFLISTIQ QLSFVQRFSD RFINFFSPYV

70 80 90 100 110 120
EISFSQYEDY QFNHAFAAIE TYLGAKATDK AKHLRASQVK ESKGLVLKRD ETKVRDEYEG

130 140 150 160 170 180
GTVWWEMETD STGYRTFKLT FHRRSRDIVT DSYIKYVFEE GKSIQAKSKQ MKLFTNNPSS

190 200 210 220 230 240
HWGTSKKSFW RYIDFEHPAS FHTLAMDTKK KEEILNDLAA FSNGKEYYKK IGKAWKRGYL

250 260 270 280 290 300
LHGPPGTGKS TMIAAMANHL NYSIYDLELT AIRNNSELRK LLTATSSKSI IVIEDIDCSL

310 320 330 340 350 360
DLTGKRKKEK NLMTSREDGE QGTEEDKSFV TLSGLLNFID GIWSACGQER IIIFTTNHFE

370 380 390 400 410 420
KLDPALIRRG RMDMHIELSY CSFEAFKILA KNYLDLDTHP LFKKIESLLK ETKIAPADVA

430 440 450 460 470
ENLMKKNTEI DADGSLKDLI QALEGKKKIH GAQVDEPKDK YTKKFYKAFC MSSKA


References and documentation are available.

Number of amino acids: 475 Molecular weight: 54609.45 Theoretical pI: 8.98
Amino acid composition: 
Ala (A) 33 6.9% Arg (R) 20 4.2% Asn (N) 18 3.8% Asp (D) 28 5.9% Cys (C) 4 0.8% Gln (Q) 14 2.9% Glu (E) 34 7.2% Gly (G) 25 5.3% His (H) 13 2.7% Ile (I) 35 7.4% Leu (L) 42 8.8% Lys (K) 51 10.7% Met (M) 11 2.3% Phe (F) 30 6.3% Pro (P) 10 2.1% Ser (S) 39 8.2% Thr (T) 31 6.5% Trp (W) 7 1.5% Tyr (Y) 18 3.8% Val (V) 12 2.5% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 62 Total number of positively charged residues (Arg + Lys): 71 Atomic composition: Carbon C 2466 Hydrogen H 3841 Nitrogen N 651 Oxygen O 720 Sulfur S 15 Formula: C2466H3841N651O720S15 Total number of atoms: 7693 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 65570 Abs 0.1% (=1 g/l) 1.201, assuming all pairs of Cys residues form cystines Ext. coefficient 65320 Abs 0.1% (=1 g/l) 1.196, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 30.75 This classifies the protein as stable. Aliphatic index: 77.49 Grand average of hydropathicity (GRAVY): -0.476