ProtParam
User-provided sequence:
10 20 30 40 50 60
MGPISSSWSF NKFFSIVFVV FAISGEFVAG YYRPGPWRYA HATFYGDETG GETMGGACGY
70 80 90 100 110 120
GNLFNSGYGL STAALSTTLF NDGYGCGQCF QITCSKSPHC YSGKSTVVTA TNLCPPNWYQ
130 140 150 160 170 180
DSNAGGWCNP PRTHFDMAKP AFMKLAYWRA GIIPVAYRRV PCQRSGGMRF QFQGNSYWLL
190 200 210 220 230 240
IFVMNVGGAG DIKSMAVKGS RTNWISMSHN WGASYQAFSS LYGQSLSFRV TSYTTGETIY
250 260
AWNVAPANWS GGKTYKSTAN FR
References and
documentation are available.
Number of amino acids: 262
Molecular weight: 28791.46
Theoretical pI: 9.33
Total number of negatively charged residues (Asp + Glu): 9
Total number of positively charged residues (Arg + Lys): 20
Atomic composition:
Carbon C 1304
Hydrogen H 1897
Nitrogen N 345
Oxygen O 367
Sulfur S 16
Formula: C
1304H
1897N
345O
367S
16
Total number of atoms: 3929
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 80830
Abs 0.1% (=1 g/l) 2.807, assuming all pairs of Cys residues form cystines
Ext. coefficient 80330
Abs 0.1% (=1 g/l) 2.790, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 24.16
This classifies the protein as stable.
Aliphatic index: 52.56
Grand average of hydropathicity (GRAVY): -0.171