ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGPISSSWSF NKFFSIVFVV FAISGEFVAG YYRPGPWRYA HATFYGDETG GETMGGACGY 

        70         80         90        100        110        120 
GNLFNSGYGL STAALSTTLF NDGYGCGQCF QITCSKSPHC YSGKSTVVTA TNLCPPNWYQ 

       130        140        150        160        170        180 
DSNAGGWCNP PRTHFDMAKP AFMKLAYWRA GIIPVAYRRV PCQRSGGMRF QFQGNSYWLL 

       190        200        210        220        230        240 
IFVMNVGGAG DIKSMAVKGS RTNWISMSHN WGASYQAFSS LYGQSLSFRV TSYTTGETIY 

       250        260 
AWNVAPANWS GGKTYKSTAN FR 

References and documentation are available.

Number of amino acids: 262

Molecular weight: 28791.46

Theoretical pI: 9.33

Amino acid composition: 
Ala (A)  22	  8.4%
Arg (R)  11	  4.2%
Asn (N)  15	  5.7%
Asp (D)   5	  1.9%
Cys (C)   8	  3.1%
Gln (Q)   8	  3.1%
Glu (E)   4	  1.5%
Gly (G)  32	 12.2%
His (H)   4	  1.5%
Ile (I)  10	  3.8%
Leu (L)  10	  3.8%
Lys (K)   9	  3.4%
Met (M)   8	  3.1%
Phe (F)  18	  6.9%
Pro (P)  12	  4.6%
Ser (S)  28	 10.7%
Thr (T)  18	  6.9%
Trp (W)  10	  3.8%
Tyr (Y)  17	  6.5%
Val (V)  13	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 9
Total number of positively charged residues (Arg + Lys): 20

Atomic composition:

Carbon      C	      1304
Hydrogen    H	      1897
Nitrogen    N	       345
Oxygen      O	       367
Sulfur      S	        16

Formula: C1304H1897N345O367S16
Total number of atoms: 3929

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    80830
Abs 0.1% (=1 g/l)   2.807, assuming all pairs of Cys residues form cystines


Ext. coefficient    80330
Abs 0.1% (=1 g/l)   2.790, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 24.16
This classifies the protein as stable.



Aliphatic index: 52.56

Grand average of hydropathicity (GRAVY): -0.171