ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGPSAHLISA LGVIIMATMV AAYEPETYAS PPPLYSSPLP EVEYKTPPLP YVDSSPPPTY 

        70         80         90        100        110        120 
TPAPEVEYKS PPPPYVYSSP PPPTYSPSPK VDYKSPPPPY VYSSPPPPYY SPSPKVDYKS 

       130        140        150        160        170        180 
PPPPYVYNSP PPPYYSPSPK VDYKSPPPPY VYSSPPPPYY SPSPKVEYKS PPPPYVYSSP 

       190        200        210        220        230        240 
PPPYYSPSPK VDYKSPPPPY VYSSPPPPYY SPSPKVEYKS PPPPYVYSSP PPPYYSPSPK 

       250        260        270        280        290        300 
VDYKSPPPPY VYSSPPPPYY SPSPKVDYKS PPPPYVYSSP PPPYYSPSPK VDYKSPPPPY 

       310        320        330        340        350        360 
VYSSPPPPYY SPSPKVDYKS PPPPYVYSSP PPPYYSPSPK VDYKSPPPPY VYSSPPPPTY 

       370        380        390        400        410        420 
SPSPKVDYKS PPPPYVYSSP PPPYYSPSPK VEYKSPPPPY VYSSPPPPTY SPSPKVYYKS 

       430        440        450        460        470        480 
PPPPYVYSSP PPPYYSPSPK VYYKSPPPPY VYSSPPPPYY SPSPKVYYKS PPPPYVYSSP 

       490        500        510        520        530        540 
PPPYYSPSPK VYYKSPPPPY VYSSPPPPYY SPSPKVYYKS PPPPYVYSSP PPPYYSPSPK 

       550        560        570        580        590        600 
VHYKSPPPPY VYSSPPPPYY SPSPKVHYKS PPPPYVYNSP PPPYYSPSPK VYYKSPPPPY 

       610        620        630        640        650        660 
VYSSPPPPYY SPSPKVYYKS PPPPYVYSSP PPPYYSPSPK VYYKSPPPPY YSPSPKVYYK 

       670        680        690        700        710        720 
SPPHPHVCVC PPPPPCYSPS PKVVYKSPPP PYVYNSPPPP YYSPSPKVYY KSPPPPSYYS 

       730        740 
PSPKVEYKSP PPPSYSPSPK TEY 

References and documentation are available.

Number of amino acids: 743

Molecular weight: 83015.06

Theoretical pI: 9.28

Amino acid composition: 
Ala (A)   7	  0.9%
Arg (R)   0	  0.0%
Asn (N)   3	  0.4%
Asp (D)  11	  1.5%
Cys (C)   3	  0.4%
Gln (Q)   0	  0.0%
Glu (E)  11	  1.5%
Gly (G)   2	  0.3%
His (H)   5	  0.7%
Ile (I)   3	  0.4%
Leu (L)   5	  0.7%
Lys (K)  57	  7.7%
Met (M)   3	  0.4%
Phe (F)   0	  0.0%
Pro (P) 283	 38.1%
Ser (S) 138	 18.6%
Thr (T)   9	  1.2%
Trp (W)   0	  0.0%
Tyr (Y) 144	 19.4%
Val (V)  59	  7.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 57

Atomic composition:

Carbon      C	      4036
Hydrogen    H	      5603
Nitrogen    N	       813
Oxygen      O	      1082
Sulfur      S	         6

Formula: C4036H5603N813O1082S6
Total number of atoms: 11540

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   214685
Abs 0.1% (=1 g/l)   2.586, assuming all pairs of Cys residues form cystines


Ext. coefficient   214560
Abs 0.1% (=1 g/l)   2.585, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 132.37
This classifies the protein as unstable.



Aliphatic index: 28.17

Grand average of hydropathicity (GRAVY): -1.046