ProtParam
User-provided sequence:
10 20 30 40 50 60
MGQILSNPII DKEHHSGTDC LTAFGLCAMQ GWRMSMEDAH IVEPNLLAES DEEHLAFYGI
70 80 90 100 110 120
FDGHGGSSVA EFCGSKMISI LKKQESFKSG MLEQCLIDTF LATDVELLKD EKLKDDHSGC
130 140 150 160 170 180
TATVILVSQL KKLLICANSG DSRTVLSTGG NSKAMSFDHK PTLLSEKSRI VAADGFVEMD
190 200 210 220 230 240
RVNGNLALSR AIGDFEFKSN TKLGPHEQVV TCVPDIICHN LNYDEDEFVI LACDGIWDCL
250 260 270 280 290 300
TSQECVDLVH YGISQGNMTL SDISSRIVDV CCSPTTEGSG IGCDNMSISI VALLKENESE
310 320 330 340 350 360
SQWFERMRSK NYNIQTSFVQ RRKSIFDFHD FSDDDNEVFA ITTKKLQDRL NRSKDNDDME
370 380 390 400 410 420
IDDLDTELDS SATPSKLSGE DRTGPIDLFS LEALLEAGIQ IRQRPSSDSD GNTSYFHGAS
430 440 450 460
LSDMLASLSN AAAGETEPND ADDNDDNDGE ENGKNENAKK GSKIEEIE
References and
documentation are available.
Number of amino acids: 468
Molecular weight: 51390.02
Theoretical pI: 4.45
Total number of negatively charged residues (Asp + Glu): 85
Total number of positively charged residues (Arg + Lys): 41
Atomic composition:
Carbon C 2201
Hydrogen H 3475
Nitrogen N 605
Oxygen O 757
Sulfur S 27
Formula: C
2201H
3475N
605O
757S
27
Total number of atoms: 7065
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 24825
Abs 0.1% (=1 g/l) 0.483, assuming all pairs of Cys residues form cystines
Ext. coefficient 23950
Abs 0.1% (=1 g/l) 0.466, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.16
This classifies the protein as unstable.
Aliphatic index: 79.00
Grand average of hydropathicity (GRAVY): -0.410