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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGQKSSKVHI TKTDRAILEV KRSKDEIHKF TRRTDNLILV EKSQLKDLIR KNPENYKSNM

70 80 90 100 110 120
KVRFLLKRIH YQEHLLQQAS DQLINLENMV STLEFKMVEK QFINGLKNGN EILKKLNKEF

130 140 150 160 170 180
SNVDELMDDV QDQIAYQNEI NETLSRSLVG TSNYEDDLDK ELDALESELN PEKMNNAKVA

190 200 210 220
NMPSTEGLPS LPQGEQTEQK EREEFATEER SDTKEPLALL S


References and documentation are available.

Number of amino acids: 221 Molecular weight: 25637.93 Theoretical pI: 5.24
Amino acid composition: 
Ala (A) 8 3.6% Arg (R) 10 4.5% Asn (N) 18 8.1% Asp (D) 14 6.3% Cys (C) 0 0.0% Gln (Q) 13 5.9% Glu (E) 27 12.2% Gly (G) 6 2.7% His (H) 4 1.8% Ile (I) 11 5.0% Leu (L) 27 12.2% Lys (K) 23 10.4% Met (M) 7 3.2% Phe (F) 6 2.7% Pro (P) 6 2.7% Ser (S) 16 7.2% Thr (T) 11 5.0% Trp (W) 0 0.0% Tyr (Y) 4 1.8% Val (V) 10 4.5% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 41 Total number of positively charged residues (Arg + Lys): 33 Atomic composition: Carbon C 1111 Hydrogen H 1815 Nitrogen N 313 Oxygen O 366 Sulfur S 7 Formula: C1111H1815N313O366S7 Total number of atoms: 3612 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 5960 Abs 0.1% (=1 g/l) 0.232 Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 53.26 This classifies the protein as unstable. Aliphatic index: 83.80 Grand average of hydropathicity (GRAVY): -0.899