ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGQQFSWEEA EASGEMDVAE LQEWYKKFVV ECPSGTLFMH EFKRFFKVTG NEEATQYVEG 

        70         80         90        100        110        120 
MFRAFDKNGD NTIDFLEYVA ALNLVLRGTL EHKLKWTFKI YDKDRNGCID RLELLDIVEA 

       130        140        150        160        170        180 
IYKLKKACRA ELDLEQQGQL LTPEEVVDRI FLLVDENGDG QLSLTEFIEG ARRDKWVMKM 

       190        200 
LQMDVNPGGW ITQQRRRSAM F 

References and documentation are available.

Number of amino acids: 201

Molecular weight: 23498.84

Theoretical pI: 4.86

Amino acid composition: 
Ala (A)  12	  6.0%
Arg (R)  12	  6.0%
Asn (N)   7	  3.5%
Asp (D)  14	  7.0%
Cys (C)   3	  1.5%
Gln (Q)  11	  5.5%
Glu (E)  22	 10.9%
Gly (G)  14	  7.0%
His (H)   2	  1.0%
Ile (I)   8	  4.0%
Leu (L)  21	 10.4%
Lys (K)  14	  7.0%
Met (M)   8	  4.0%
Phe (F)  13	  6.5%
Pro (P)   3	  1.5%
Ser (S)   5	  2.5%
Thr (T)   9	  4.5%
Trp (W)   5	  2.5%
Tyr (Y)   5	  2.5%
Val (V)  13	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 26

Atomic composition:

Carbon      C	      1052
Hydrogen    H	      1628
Nitrogen    N	       278
Oxygen      O	       311
Sulfur      S	        11

Formula: C1052H1628N278O311S11
Total number of atoms: 3280

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    35075
Abs 0.1% (=1 g/l)   1.493, assuming all pairs of Cys residues form cystines


Ext. coefficient    34950
Abs 0.1% (=1 g/l)   1.487, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.96
This classifies the protein as stable.



Aliphatic index: 81.00

Grand average of hydropathicity (GRAVY): -0.421