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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGQQVLKFSN EKMEKAVAAY SRELATVRAG RASASVLDKV QVDYYGAPTP VVQLANITVP

70 80 90 100 110 120
EARLLVIQPY DKTSIGDIEK AILKADLGLN PSNDGTVIRI AFPALTEERR RDLVKVVKKY

130 140 150 160 170 180
AEEAKVAVRN VRRDGNDDLK KLEKAGEITE DDLRGYTEDI QKETDKYIAK VDEIAKNKEK


EIMEV


References and documentation are available.

Number of amino acids: 185 Molecular weight: 20679.65 Theoretical pI: 5.79
Amino acid composition: 
Ala (A) 20 10.8% Arg (R) 12 6.5% Asn (N) 7 3.8% Asp (D) 15 8.1% Cys (C) 0 0.0% Gln (Q) 6 3.2% Glu (E) 18 9.7% Gly (G) 9 4.9% His (H) 0 0.0% Ile (I) 12 6.5% Leu (L) 14 7.6% Lys (K) 20 10.8% Met (M) 3 1.6% Phe (F) 2 1.1% Pro (P) 6 3.2% Ser (S) 6 3.2% Thr (T) 9 4.9% Trp (W) 0 0.0% Tyr (Y) 7 3.8% Val (V) 19 10.3% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 33 Total number of positively charged residues (Arg + Lys): 32 Atomic composition: Carbon C 909 Hydrogen H 1504 Nitrogen N 254 Oxygen O 287 Sulfur S 3 Formula: C909H1504N254O287S3 Total number of atoms: 2957 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 10430 Abs 0.1% (=1 g/l) 0.504 Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 43.13 This classifies the protein as unstable. Aliphatic index: 95.41 Grand average of hydropathicity (GRAVY): -0.498