ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGQTSSHRQM HHSSNNNNKE EPPQPASQFG NRRTSTRKST SSSKHYPTLV QPLRKDELSL 

        70         80         90        100        110        120 
LRDSLLRNGS IMRKNPERRP VTISELKPVN TGQGTSGSVL PITAEDAAGG SSSSITQLMA 

       130        140        150        160        170        180 
FQLPVDRSAK TDDLAAESSA SAAAAVAPKE GRNEFYIVGD GYHRTDHCYY RSPNGDFHKL 

       190        200        210        220        230        240 
PSDSFHKMSE GCFVRMADGV FRRLEVPTKA QISRRSSRAE EIGDATSQGN GSAATNVKSQ 

       250        260        270        280        290        300 
LMRFLKRSKS HTPATIAKLQ REKEEARAER KQRLSNALRQ KNLAATNAAS AAAANSSYAS 

       310        320        330        340        350        360 
GGGGGTVSSR NNKIVVTMIE NGGLPIVATS KAERPKAKES ASSSDKARNS RGSPNMQMRS 

       370        380        390        400        410        420 
SAPMRWRATE EHIGKYKLIK TIGKGNFAKV KLAKHLPTGK EVAIKIIDKT QLNPGSLQKL 

       430        440        450        460        470        480 
FREVRIMKML DHPNIVKLFQ VIETEKTLYL IMEYASGGEV FDYLVLHGRM KEKEARVKFR 

       490        500        510        520        530        540 
QIVSAVQYCH QKRIIHRDLK AENLLLDSEL NIKIADFGFS NEFTPGSKLD TFCGSPPYAA 

       550        560        570        580        590        600 
PELFQGKKYD GPEVDVWSLG VILYTLVSGS LPFDGSTLRE LRERVLRGKY RIPFYMSTDC 

       610        620        630        640        650        660 
ENLLRKFLVL NPAKRASLET IMGDKWMNMG FEEDELKPYI EPKADLADPK RIEALVAMGY 

       670        680        690        700        710        720 
NRSEIEASLS QVRYDDVFAT YLLLGRKSTD PESDGSRSGS SLSLRNISGN DAGANAGSAS 

       730        740        750        760        770        780 
VQSPTHRGVH RSISASSTKP SRRASSGAET LRVGPTNAAA TVAAATGAVG AVNPSNNYNA 

       790        800        810        820        830        840 
AGSAADRASV GSNFKRQNTI DSATIKENTA RLAAQNQRPA SATQKMLTTA DTTLNSPAKP 

       850        860        870        880        890        900 
RTATKYDPTN GNRTVSGTSG IIPRRSTTLY EKTSSTEKTN VIPAETNSGS AAPAGKGHTK 

       910        920        930        940        950        960 
SASVSSPRPS TDGCVSVSND PLGTRMASAV KSSRHFPRNV PSRSTFHSGQ TRARNNTALE 

       970        980        990 
YSGTSGASGD SSHPGRMSFF SKLSSRFSKR PNQ 

References and documentation are available.

Number of amino acids: 993

Molecular weight: 107760.13

Theoretical pI: 9.97

Amino acid composition: 
Ala (A)  98	  9.9%
Arg (R)  77	  7.8%
Asn (N)  53	  5.3%
Asp (D)  40	  4.0%
Cys (C)   6	  0.6%
Gln (Q)  30	  3.0%
Glu (E)  53	  5.3%
Gly (G)  71	  7.2%
His (H)  21	  2.1%
Ile (I)  39	  3.9%
Leu (L)  69	  6.9%
Lys (K)  67	  6.7%
Met (M)  23	  2.3%
Phe (F)  29	  2.9%
Pro (P)  52	  5.2%
Ser (S) 123	 12.4%
Thr (T)  67	  6.7%
Trp (W)   3	  0.3%
Tyr (Y)  24	  2.4%
Val (V)  48	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 93
Total number of positively charged residues (Arg + Lys): 144

Atomic composition:

Carbon      C	      4641
Hydrogen    H	      7521
Nitrogen    N	      1419
Oxygen      O	      1477
Sulfur      S	        29

Formula: C4641H7521N1419O1477S29
Total number of atoms: 15087

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    52635
Abs 0.1% (=1 g/l)   0.488, assuming all pairs of Cys residues form cystines


Ext. coefficient    52260
Abs 0.1% (=1 g/l)   0.485, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.11
This classifies the protein as unstable.



Aliphatic index: 66.30

Grand average of hydropathicity (GRAVY): -0.631