ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGRSRGVQNS GDDDTVHRSK RRRVASGGDA TDSVSAGIGG AGEGGGKKAL YHCNYCNKDI 

        70         80         90        100        110        120 
SGKIRIKCSK CPDFDLCVEC FSVGAEVTPH RSNHPYKVMD NLSFPLICPD WNADEEILLL 

       130        140        150        160        170        180 
EGIEMYGLGN WLEVAEHVGT KSKLQCIDHY TTAYMNSPCY PLPDMSHVNG KNRKELLAMA 

       190        200        210        220        230        240 
KVQGESKKGT SLLPGELTPK AESPFSPSRV KVEDALGEGL AGRSPSHIAV GANKKASNVG 

       250        260        270        280        290        300 
HIKDGSNVSK VEDGHVDRSV GVKKPRYSAD EGPSLTELSG YNAKRHEFDP EYDNDAEQAL 

       310        320        330        340        350        360 
AEMEFKETDS ETDRELKLRV LRIYLSRLDE RKRRKEFILE RNLLFPNPLE KDLTNEDREV 

       370        380        390        400        410        420 
YHRYKVFMRF LSKEEHEALV RSVIEERKIR RRIQELQECR SAGCRTLAEA KIHIEQKRKK 

       430        440        450        460        470        480 
EYELNAQKAK ESNHLIANTK LVQKMNRPMK IESDGNLDPK KGGVALDSPK TTGLTSVKQW 

       490        500        510        520        530        540 
DDWDIVGLPG AKLLSASEKL LCCQNRLLPS HYLRMQEVLM QEIFKGSVLK KEDAHVLFKV 

       550        560 
DPTKVDSVYD MVTKKLGNHV ELPTV 

References and documentation are available.

Number of amino acids: 565

Molecular weight: 63461.22

Theoretical pI: 8.25

Amino acid composition: 
Ala (A)  33	  5.8%
Arg (R)  36	  6.4%
Asn (N)  25	  4.4%
Asp (D)  36	  6.4%
Cys (C)  13	  2.3%
Gln (Q)  13	  2.3%
Glu (E)  50	  8.8%
Gly (G)  41	  7.3%
His (H)  18	  3.2%
Ile (I)  21	  3.7%
Leu (L)  54	  9.6%
Lys (K)  54	  9.6%
Met (M)  13	  2.3%
Phe (F)  12	  2.1%
Pro (P)  25	  4.4%
Ser (S)  42	  7.4%
Thr (T)  20	  3.5%
Trp (W)   4	  0.7%
Tyr (Y)  16	  2.8%
Val (V)  39	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 86
Total number of positively charged residues (Arg + Lys): 90

Atomic composition:

Carbon      C	      2764
Hydrogen    H	      4455
Nitrogen    N	       805
Oxygen      O	       854
Sulfur      S	        26

Formula: C2764H4455N805O854S26
Total number of atoms: 8904

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    46590
Abs 0.1% (=1 g/l)   0.734, assuming all pairs of Cys residues form cystines


Ext. coefficient    45840
Abs 0.1% (=1 g/l)   0.722, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.40
This classifies the protein as unstable.



Aliphatic index: 77.63

Grand average of hydropathicity (GRAVY): -0.671