ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGSANNEYEF LSEIGLTSHN LGSYVAGKWQ ANGPLVSTLN PANNQPIAQV VEASLEDYEQ 

        70         80         90        100        110        120 
GLKACEEAAK IWMQVTAPKR GDIVRQIGDA LRSKLDYLGR LLSLEMGKIL AEGIGEVQEV 

       130        140        150        160        170        180 
IDMCDFAVGL SRQLNGSVIP SERPNHMMLE MWNPLGIVGV ITAFNFPCAV LGWNACIALV 

       190        200        210        220        230        240 
CGNCVVWKGA PTTPLITIAM TKLVAEVLEK NNLPGAIFTA MCGGAEIGEA IAKDTRIPLV 

       250        260        270        280        290        300 
SFTGSSRVGS MVQQTVNARS GKTLLELSGN NAIIVMDDAD IQLAARSVLF AAVGTAGQRC 

       310        320        330        340        350        360 
TTCRRLLLHE SVYDKVLEQL LTSYKQVKIG NPLEKGTLLG PLHTPESKKN FEKGIEVIKS 

       370        380        390        400        410        420 
QGGKILTGGK AVEGEGNFVE PTIIEISADA AVVKEELFAP VLYVLKFKSF GEAVAINNSV 

       430        440        450        460        470        480 
PQGLSSSIFT RNPENIFRWI GPLGSDCGIV NVNIPTNGAE IGGAFGGEKA TGGGREAGSD 

       490        500 
SWKQYMRRST CTINYGNELP LAQGINFG 

References and documentation are available.

Number of amino acids: 508

Molecular weight: 54208.28

Theoretical pI: 5.45

Amino acid composition: 
Ala (A)  45	  8.9%
Arg (R)  18	  3.5%
Asn (N)  31	  6.1%
Asp (D)  14	  2.8%
Cys (C)  11	  2.2%
Gln (Q)  18	  3.5%
Glu (E)  37	  7.3%
Gly (G)  56	 11.0%
His (H)   4	  0.8%
Ile (I)  37	  7.3%
Leu (L)  48	  9.4%
Lys (K)  26	  5.1%
Met (M)  12	  2.4%
Phe (F)  16	  3.1%
Pro (P)  22	  4.3%
Ser (S)  32	  6.3%
Thr (T)  26	  5.1%
Trp (W)   7	  1.4%
Tyr (Y)   9	  1.8%
Val (V)  39	  7.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 44

Atomic composition:

Carbon      C	      2400
Hydrogen    H	      3850
Nitrogen    N	       652
Oxygen      O	       727
Sulfur      S	        23

Formula: C2400H3850N652O727S23
Total number of atoms: 7652

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    52535
Abs 0.1% (=1 g/l)   0.969, assuming all pairs of Cys residues form cystines


Ext. coefficient    51910
Abs 0.1% (=1 g/l)   0.958, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.79
This classifies the protein as stable.



Aliphatic index: 96.38

Grand average of hydropathicity (GRAVY): 0.048