ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGSCCSCLKD SSDEASVSPI ADNEREAVTL LLGYLEDKDQ LDFYSGGPLK ALTTLVYSDN 

        70         80         90        100        110        120 
LNLQRSAALA FAEITEKYVR QVSREVLEPI LILLQSQDPQ IQVAACAALG NLAVNNENKL 

       130        140        150        160        170        180 
LIVEMGGLEP LINQMMGDNV EVQCNAVGCI TNLATRDDNK HKIATSGALI PLTKLAKSKH 

       190        200        210        220        230        240 
IRVQRNATGA LLNMTHSEEN RKELVNAGAV PVLVSLLSST DPDVQYYCTT ALSNIAVDEA 

       250        260        270        280        290        300 
NRKKLAQTEP RLVSKLVSLM DSPSSRVKCQ ATLALRNLAS DTSYQLEIVR AGGLPHLVKL 

       310        320        330        340        350        360 
IQSDSIPLVL ASVACIRNIS IHPLNEGLIV DAGFLKPLVR LLDYKDSEEI QCHAVSTLRN 

       370        380        390        400        410        420 
LAASSEKNRK EFFESGAVEK CKELALDSPV SVQSEISACF AILALADVSK LDLLEANILD 

       430        440        450        460        470        480 
ALIPMTFSQN QEVSGNAAAA LANLCSRVNN YTKIIEAWDR PNEGIRGFLI RFLKSDYATF 

       490        500        510        520        530        540 
EHIALWTILQ LLESHNDKVE DLVKNDDDII NGVRKMADVT FERLQRSGID VKNPGSNNNP 

       550        560        570 
SSNDNNSNNN DTGSEHQPVE DASLELYNIT QQILQFLH 

References and documentation are available.

Number of amino acids: 578

Molecular weight: 63207.85

Theoretical pI: 5.00

Amino acid composition: 
Ala (A)  54	  9.3%
Arg (R)  24	  4.2%
Asn (N)  44	  7.6%
Asp (D)  36	  6.2%
Cys (C)  13	  2.2%
Gln (Q)  25	  4.3%
Glu (E)  38	  6.6%
Gly (G)  24	  4.2%
His (H)  10	  1.7%
Ile (I)  35	  6.1%
Leu (L)  77	 13.3%
Lys (K)  29	  5.0%
Met (M)   8	  1.4%
Phe (F)  12	  2.1%
Pro (P)  20	  3.5%
Ser (S)  51	  8.8%
Thr (T)  24	  4.2%
Trp (W)   2	  0.3%
Tyr (Y)  11	  1.9%
Val (V)  41	  7.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 74
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      2757
Hydrogen    H	      4488
Nitrogen    N	       770
Oxygen      O	       882
Sulfur      S	        21

Formula: C2757H4488N770O882S21
Total number of atoms: 8918

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    28140
Abs 0.1% (=1 g/l)   0.445, assuming all pairs of Cys residues form cystines


Ext. coefficient    27390
Abs 0.1% (=1 g/l)   0.433, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.84
This classifies the protein as unstable.



Aliphatic index: 105.48

Grand average of hydropathicity (GRAVY): -0.118