ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGSCFSSRVK ADIFHNGKSS DLYGLSLSSR KSSSTVAAAQ KTEGEILSST PVKSFTFNEL 

        70         80         90        100        110        120 
KLATRNFRPD SVIGEGGFGC VFKGWLDEST LTPTKPGTGL VIAVKKLNQE GFQGHREWLT 

       130        140        150        160        170        180 
EINYLGQLSH PNLVKLIGYC LEDEHRLLVY EFMQKGSLEN HLFRRGAYFK PLPWFLRVNV 

       190        200        210        220        230        240 
ALDAAKGLAF LHSDPVKVIY RDIKASNILL DADYNAKLSD FGLARDGPMG DLSYVSTRVM 

       250        260        270        280        290        300 
GTYGYAAPEY MSSGHLNARS DVYSFGVLLL EILSGKRALD HNRPAKEENL VDWARPYLTS 

       310        320        330        340        350        360 
KRKVLLIVDN RLDTQYLPEE AVRMASVAVQ CLSFEPKSRP TMDQVVRALQ QLQDNLGKPS 

       370        380        390 
QTNPVKDTKK LGFKTGTTKS SEKRFTQKPF GRHLV 

References and documentation are available.

Number of amino acids: 395

Molecular weight: 44097.48

Theoretical pI: 9.36

Amino acid composition: 
Ala (A)  25	  6.3%
Arg (R)  23	  5.8%
Asn (N)  16	  4.1%
Asp (D)  21	  5.3%
Cys (C)   4	  1.0%
Gln (Q)  13	  3.3%
Glu (E)  20	  5.1%
Gly (G)  30	  7.6%
His (H)   9	  2.3%
Ile (I)  11	  2.8%
Leu (L)  48	 12.2%
Lys (K)  30	  7.6%
Met (M)   7	  1.8%
Phe (F)  19	  4.8%
Pro (P)  18	  4.6%
Ser (S)  35	  8.9%
Thr (T)  21	  5.3%
Trp (W)   4	  1.0%
Tyr (Y)  14	  3.5%
Val (V)  27	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      1976
Hydrogen    H	      3121
Nitrogen    N	       545
Oxygen      O	       577
Sulfur      S	        11

Formula: C1976H3121N545O577S11
Total number of atoms: 6230

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    43110
Abs 0.1% (=1 g/l)   0.978, assuming all pairs of Cys residues form cystines


Ext. coefficient    42860
Abs 0.1% (=1 g/l)   0.972, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.39
This classifies the protein as stable.



Aliphatic index: 84.41

Grand average of hydropathicity (GRAVY): -0.336