ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGSETKHSAK VKIVTRESPP SAKEHMRPTK TQILVPPTQS LPNGKKPNFG KSTKQRREPR 

        70         80         90        100        110        120 
ERTSKTGHED DKATMVTVNI DAFLHDKAPK KKSCKYKKKK TRQYQDRAAA SIDSKPHVAG 

       130        140 
HTAFAGASFT TDIPHEAALP KPSFV 

References and documentation are available.

Number of amino acids: 145

Molecular weight: 16071.38

Theoretical pI: 10.14

Amino acid composition: 
Ala (A)  14	  9.7%
Arg (R)   8	  5.5%
Asn (N)   3	  2.1%
Asp (D)   7	  4.8%
Cys (C)   1	  0.7%
Gln (Q)   5	  3.4%
Glu (E)   7	  4.8%
Gly (G)   6	  4.1%
His (H)   7	  4.8%
Ile (I)   5	  3.4%
Leu (L)   4	  2.8%
Lys (K)  22	 15.2%
Met (M)   3	  2.1%
Phe (F)   5	  3.4%
Pro (P)  13	  9.0%
Ser (S)  12	  8.3%
Thr (T)  14	  9.7%
Trp (W)   0	  0.0%
Tyr (Y)   2	  1.4%
Val (V)   7	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 14
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	       703
Hydrogen    H	      1147
Nitrogen    N	       213
Oxygen      O	       210
Sulfur      S	         4

Formula: C703H1147N213O210S4
Total number of atoms: 2277

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     2980
Abs 0.1% (=1 g/l)   0.185, assuming all pairs of Cys residues form cystines


Ext. coefficient     2980
Abs 0.1% (=1 g/l)   0.185, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.80
This classifies the protein as stable.



Aliphatic index: 47.86

Grand average of hydropathicity (GRAVY): -1.048