ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGSFLAAFRF LTNIPVPWLK EDWQGEKSHL DFARSLGFYP LVGLIVGLIL AVLGWVFSLF 

        70         80         90        100        110        120 
LPDVLTAALL TVSLVVITGG LHLDGLTDTF DGIAAGHKSF ERAQEVMHLP GVGAIGVLAA 

       130        140        150        160        170        180 
ICLLLSKFAA IVSLPSGSFI TGLILFPVIS RWAMVYAVVC YPYVRSQGLG KELKGGSAKA 

       190        200        210        220        230        240 
SLWLATIFCL AVCVILGGWA GLLAMFGSWL LIMYLASFFR RRLGGLNGDT YGSINEFTEV 

       250 
AALIFIVALS GYFS 

References and documentation are available.

Number of amino acids: 254

Molecular weight: 27262.37

Theoretical pI: 7.69

Amino acid composition: 
Ala (A)  26	 10.2%
Arg (R)   8	  3.1%
Asn (N)   3	  1.2%
Asp (D)   7	  2.8%
Cys (C)   4	  1.6%
Gln (Q)   3	  1.2%
Glu (E)   7	  2.8%
Gly (G)  29	 11.4%
His (H)   4	  1.6%
Ile (I)  17	  6.7%
Leu (L)  42	 16.5%
Lys (K)   7	  2.8%
Met (M)   5	  2.0%
Phe (F)  19	  7.5%
Pro (P)   8	  3.1%
Ser (S)  19	  7.5%
Thr (T)  10	  3.9%
Trp (W)   7	  2.8%
Tyr (Y)   7	  2.8%
Val (V)  22	  8.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 14
Total number of positively charged residues (Arg + Lys): 15

Atomic composition:

Carbon      C	      1289
Hydrogen    H	      1990
Nitrogen    N	       306
Oxygen      O	       325
Sulfur      S	         9

Formula: C1289H1990N306O325S9
Total number of atoms: 3919

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    49180
Abs 0.1% (=1 g/l)   1.804, assuming all pairs of Cys residues form cystines


Ext. coefficient    48930
Abs 0.1% (=1 g/l)   1.795, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.20
This classifies the protein as stable.



Aliphatic index: 125.94

Grand average of hydropathicity (GRAVY): 0.944