ProtParam
User-provided sequence:
10 20 30 40 50 60
MGSFLAAFRF LTNIPVPWLK EDWQGEKSHL DFARSLGFYP LVGLIVGLIL AVLGWVFSLF
70 80 90 100 110 120
LPDVLTAALL TVSLVVITGG LHLDGLTDTF DGIAAGHKSF ERAQEVMHLP GVGAIGVLAA
130 140 150 160 170 180
ICLLLSKFAA IVSLPSGSFI TGLILFPVIS RWAMVYAVVC YPYVRSQGLG KELKGGSAKA
190 200 210 220 230 240
SLWLATIFCL AVCVILGGWA GLLAMFGSWL LIMYLASFFR RRLGGLNGDT YGSINEFTEV
250
AALIFIVALS GYFS
References and
documentation are available.
Number of amino acids: 254
Molecular weight: 27262.37
Theoretical pI: 7.69
Total number of negatively charged residues (Asp + Glu): 14
Total number of positively charged residues (Arg + Lys): 15
Atomic composition:
Carbon C 1289
Hydrogen H 1990
Nitrogen N 306
Oxygen O 325
Sulfur S 9
Formula: C
1289H
1990N
306O
325S
9
Total number of atoms: 3919
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 49180
Abs 0.1% (=1 g/l) 1.804, assuming all pairs of Cys residues form cystines
Ext. coefficient 48930
Abs 0.1% (=1 g/l) 1.795, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.20
This classifies the protein as stable.
Aliphatic index: 125.94
Grand average of hydropathicity (GRAVY): 0.944