ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGSGDTRGES SLVAKPIEII LNKLPHAILA QQQFQKYITS PIYRYLSKLL LFREVAWPES 

        70         80         90        100        110        120 
TKDTQKGQVG IFSFQNNYAD SATTFRILAH LDEHGYPLPN GAAEKNLPSL FEGFKATVSI 

       130        140        150        160        170        180 
IQQRLLLDNV DGATNSDKEK YVQLPDINTG FVNKTYSRID LTHLLEDVET NVENLSINKT 

       190        200        210        220        230        240 
LEMDELTRLD SMINELESRK LKILERVKHI DSKSTNLEND VTLIKDRINF IEEYNLEADR 

       250        260        270        280        290        300 
EQSLRKQMEE ERSSEASSFT QNEEAISSLC DVESKDTRLK DFYKMPHEKS HDKNRQIISE 

       310        320        330        340        350        360 
TYSRNTTAFR MTIPHGEHGN SITALDFDTP WGTLCSSSYQ DRIVKVWDLN HGIQVGELPG 

       370        380        390        400        410        420 
HLATVNCMQI DKKNYNMLIT GSKDATLKLW DLNLSREIYL DHSPLKEKTE EIVTPCIHNF 

       430        440        450        460        470        480 
ELHKDEITAL SFDSEALVSG SRDKKIFHWD LTTGKCIQQL DLIFTPTHSD IKMPARSLNN 

       490        500        510        520        530        540 
GACLLGTEAP MIGALQCYNS ALATGTKDGI VRLWDLRVGK PVRLLEGHTD GITSLKFDSE 

       550        560        570        580        590        600 
KLVTGSMDNS VRIWDLRTSS ILDVIAYDLP VSSLDFDGKL ITVGANEGGV NVFNMERDEH 

       610        620        630        640 
WMTPEPPHSL DGDELSRRIA IVKYKDGFLI NGHNDGDINV WTL 

References and documentation are available.

Number of amino acids: 643

Molecular weight: 72742.12

Theoretical pI: 5.26

Amino acid composition: 
Ala (A)  29	  4.5%
Arg (R)  29	  4.5%
Asn (N)  37	  5.8%
Asp (D)  50	  7.8%
Cys (C)   7	  1.1%
Gln (Q)  20	  3.1%
Glu (E)  47	  7.3%
Gly (G)  36	  5.6%
His (H)  20	  3.1%
Ile (I)  47	  7.3%
Leu (L)  71	 11.0%
Lys (K)  42	  6.5%
Met (M)  13	  2.0%
Phe (F)  21	  3.3%
Pro (P)  22	  3.4%
Ser (S)  52	  8.1%
Thr (T)  45	  7.0%
Trp (W)   9	  1.4%
Tyr (Y)  16	  2.5%
Val (V)  30	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 97
Total number of positively charged residues (Arg + Lys): 71

Atomic composition:

Carbon      C	      3210
Hydrogen    H	      5080
Nitrogen    N	       878
Oxygen      O	      1008
Sulfur      S	        20

Formula: C3210H5080N878O1008S20
Total number of atoms: 10196

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    73715
Abs 0.1% (=1 g/l)   1.013, assuming all pairs of Cys residues form cystines


Ext. coefficient    73340
Abs 0.1% (=1 g/l)   1.008, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.32
This classifies the protein as stable.



Aliphatic index: 89.61

Grand average of hydropathicity (GRAVY): -0.449