ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGSLMQSEPE MEKKPIGIRF LERIKGSKLS YSAYQAIVLI VTFLAYASYH AARKTTSIVK 

        70         80         90        100        110        120 
SALDPQSPDT GLNSLLLRFT SFGSSVKEEG GWAPFNGPDG TVLLGEIDVA FLAVYAFGMY 

       130        140        150        160        170        180 
FAGHLGDRMN LRIFLTVGMI GTGLFTSLFG VGYWGNIHSF YYFLIMQMLA GLFQSSGWPS 

       190        200        210        220        230        240 
VVAVVGNWFN KKKRGLIMGI WNAHTSVGNI TGSLIAAAML RYGWGWSFVV PGVIIVVIGL 

       250        260        270        280        290        300 
VNYAFLPVSP ENVGAERDEV LDSSSEKIGN SVNEPLLLSS SDSETDDKKR AVGFIEAWRI 

       310        320        330        340        350        360 
PGVAPFALCL FFAKLVAYTF LYWLPFYVSH TAIEGEYLSD ETAGNLSTMF DVGGVVGGIM 

       370        380        390        400        410        420 
AGYISDRIGA RAITAASFMY CSIPALFFYR SYGHVSLLAN ASLMFLTGML VNGPYALITT 

       430        440        450        460        470        480 
AVSADLGTHS SLKGNSRALA TVTAIIDGTG SVGAAVGPLL TGYISSRGSW TAVFTMLMGA 

       490        500        510        520 
AFVAGLLLTR LVMAEVAEKI AESRPSEECR SPVDYVQDHV MEV 

References and documentation are available.

Number of amino acids: 523

Molecular weight: 56309.10

Theoretical pI: 5.95

Amino acid composition: 
Ala (A)  51	  9.8%
Arg (R)  19	  3.6%
Asn (N)  16	  3.1%
Asp (D)  17	  3.3%
Cys (C)   3	  0.6%
Gln (Q)   6	  1.1%
Glu (E)  23	  4.4%
Gly (G)  55	 10.5%
His (H)   8	  1.5%
Ile (I)  32	  6.1%
Leu (L)  54	 10.3%
Lys (K)  16	  3.1%
Met (M)  19	  3.6%
Phe (F)  30	  5.7%
Pro (P)  19	  3.6%
Ser (S)  50	  9.6%
Thr (T)  28	  5.4%
Trp (W)  10	  1.9%
Tyr (Y)  22	  4.2%
Val (V)  45	  8.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 35

Atomic composition:

Carbon      C	      2578
Hydrogen    H	      3972
Nitrogen    N	       644
Oxygen      O	       726
Sulfur      S	        22

Formula: C2578H3972N644O726S22
Total number of atoms: 7942

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    87905
Abs 0.1% (=1 g/l)   1.561, assuming all pairs of Cys residues form cystines


Ext. coefficient    87780
Abs 0.1% (=1 g/l)   1.559, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.66
This classifies the protein as stable.



Aliphatic index: 98.83

Grand average of hydropathicity (GRAVY): 0.416