ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGSNKSKPKD ASQRRRSLEP AENVHGAGGA FPASQTPSKP ASADGHRGPN AAFVPPAAAE 

        70         80         90        100        110        120 
PKLFGGFNSS DTVTSPQRAG PLAGGVTTFV ALYDYESRTE TDLSFKKGER LQIVNNTRKV 

       130        140        150        160        170        180 
DVREGDWWLA HSLSTGQTGY IPSNYVAPSD SIQAEEWYFG KITRRESERL LLNAENPRGT 

       190        200        210        220        230        240 
FLVRESETTK GAYCLSVSDF DNAKGLNVKH YKIRKLDSGG FYITSRTQFN SLQQLVAYYS 

       250        260        270        280        290        300 
KHADGLCHRL TTVCPTSKPQ TQGLAKDAWE IPRESLRLEV KLGQGCFGEV WMGTWNGTTR 

       310        320        330        340        350        360 
VAIKTLKPGT MSPEAFLQEA QVMKKLRHEK LVQLYAVVSE EPIYIVTEYM NKGSLLDFLK 

       370        380        390        400        410        420 
GETGKYLRLP QLVDMSAQIA SGMAYVERMN YVHRDLRAAN ILVGENLVCK VADFGLARLI 

       430        440        450        460        470        480 
EDNEYTARQG AKFPIKWTAP EAALYGRFTI KSDVWSFGIL LTELTTKGRV PYPGMVNREV 

       490        500        510        520        530        540 
LDQVERGYRM PCPPECPESL HDLMCQCWRK EPEERPTFEY LQAFLEDYFT STEPQYQPGE 


NL 

References and documentation are available.

Number of amino acids: 542

Molecular weight: 60726.83

Theoretical pI: 7.95

Amino acid composition: 
Ala (A)  42	  7.7%
Arg (R)  34	  6.3%
Asn (N)  20	  3.7%
Asp (D)  22	  4.1%
Cys (C)   9	  1.7%
Gln (Q)  23	  4.2%
Glu (E)  42	  7.7%
Gly (G)  42	  7.7%
His (H)   9	  1.7%
Ile (I)  16	  3.0%
Leu (L)  49	  9.0%
Lys (K)  32	  5.9%
Met (M)  11	  2.0%
Phe (F)  21	  3.9%
Pro (P)  33	  6.1%
Ser (S)  36	  6.6%
Thr (T)  36	  6.6%
Trp (W)   9	  1.7%
Tyr (Y)  23	  4.2%
Val (V)  33	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 64
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      2702
Hydrogen    H	      4206
Nitrogen    N	       746
Oxygen      O	       809
Sulfur      S	        20

Formula: C2702H4206N746O809S20
Total number of atoms: 8483

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    84270
Abs 0.1% (=1 g/l)   1.388, assuming all pairs of Cys residues form cystines


Ext. coefficient    83770
Abs 0.1% (=1 g/l)   1.379, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.58
This classifies the protein as unstable.



Aliphatic index: 72.18

Grand average of hydropathicity (GRAVY): -0.495