ProtParam
User-provided sequence:
10 20 30 40 50 60
MGSNTSKVGA GAEKQQVYTP LTQIDFSQSL VSQLDSSKES DYVTKQNAEK FIEKKVSQRL
70 80 90 100 110 120
SNLEVETLKK FEDTLNNSLL SDDDKDAVDG ISSSSLNNQI ESLNKKLTLF DQLELQKLEK
130 140 150 160 170
YGGAKGKSDK KTDNGSISIK AKLTECLLAN KGKPLNCYEE MEEFKKLVMG
References and
documentation are available.
Number of amino acids: 170
Molecular weight: 18855.25
Theoretical pI: 5.40
Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 25
Atomic composition:
Carbon C 818
Hydrogen H 1342
Nitrogen N 218
Oxygen O 279
Sulfur S 5
Formula: C
818H
1342N
218O
279S
5
Total number of atoms: 2662
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 6085
Abs 0.1% (=1 g/l) 0.323, assuming all pairs of Cys residues form cystines
Ext. coefficient 5960
Abs 0.1% (=1 g/l) 0.316, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 30.62
This classifies the protein as stable.
Aliphatic index: 79.71
Grand average of hydropathicity (GRAVY): -0.724