ProtParam
User-provided sequence:
10 20 30 40 50 60
MGSRDDEYDY LFKVVLIGDS GVGKSNLLSR FTRNEFNLES KSTIGVEFAT RSISVEGKTV
70 80 90 100 110 120
KAQIWDTAGQ ERYRAITSAY YRGAVGALLV YDIAKHVTYE NVERWLKELR DHADQNIVIM
130 140 150 160 170 180
LVGNKSDLRH LRAVPTDEAK IYAERNQLSF IETSALDSTN VEAAFTNILT EIYKSVSNKH
190 200 210
VGTDRQGYGG GSGTIIPSPA SDPPKKQCCI P
References and
documentation are available.
Number of amino acids: 211
Molecular weight: 23429.35
Theoretical pI: 6.44
Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 26
Atomic composition:
Carbon C 1034
Hydrogen H 1637
Nitrogen N 289
Oxygen O 324
Sulfur S 4
Formula: C
1034H
1637N
289O
324S
4
Total number of atoms: 3288
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 26025
Abs 0.1% (=1 g/l) 1.111, assuming all pairs of Cys residues form cystines
Ext. coefficient 25900
Abs 0.1% (=1 g/l) 1.105, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.70
This classifies the protein as stable.
Aliphatic index: 85.02
Grand average of hydropathicity (GRAVY): -0.386