ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGSRLNFKSF VDGVSEQQPT VGTSLPLTRQ NSVFSLTFDE FQNSWGGGIG KDFGSMNMDE 

        70         80         90        100        110        120 
LLKNIWTAEE SHSMMGNNTS YTNISNGNSG NTVINGGGNN IGGLAVGVGG ESGGFFTGGS 

       130        140        150        160        170        180 
LQRQGSLTLP RTISQKRVDD VWKELMKEDD IGNGVVNGGT SGIPQRQQTL GEMTLEEFLV 

       190        200        210        220        230        240 
RAGVVREEPQ PVESVTNFNG GFYGFGSNGG LGTASNGFVA NQPQDLSGNG VAVRQDLLTA 

       250        260        270        280        290        300 
QTQPLQMQQP QMVQQPQMVQ QPQQLIQTQE RPFPKQTTIA FSNTVDVVNR SQPATQCQEV 

       310        320        330        340        350        360 
KPSILGIHNH PMNNNLLQAV DFKTGVTVAA VSPGSQMSPD LTPKSALDAS LSPVPYMFGR 

       370        380        390        400        410        420 
VRKTGAVLEK VIERRQKRMI KNRESAARSR ARKQAYTMEL EAEIAQLKEL NEELQKKQVE 

       430        440        450 
IMEKQKNQLL EPLRQPWGMG CKRQCLRRTL TGPW 

References and documentation are available.

Number of amino acids: 454

Molecular weight: 49677.03

Theoretical pI: 8.66

Amino acid composition: 
Ala (A)  21	  4.6%
Arg (R)  24	  5.3%
Asn (N)  31	  6.8%
Asp (D)  14	  3.1%
Cys (C)   3	  0.7%
Gln (Q)  44	  9.7%
Glu (E)  28	  6.2%
Gly (G)  49	 10.8%
His (H)   3	  0.7%
Ile (I)  16	  3.5%
Leu (L)  36	  7.9%
Lys (K)  21	  4.6%
Met (M)  17	  3.7%
Phe (F)  17	  3.7%
Pro (P)  24	  5.3%
Ser (S)  33	  7.3%
Thr (T)  30	  6.6%
Trp (W)   5	  1.1%
Tyr (Y)   4	  0.9%
Val (V)  34	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 45

Atomic composition:

Carbon      C	      2148
Hydrogen    H	      3447
Nitrogen    N	       633
Oxygen      O	       681
Sulfur      S	        20

Formula: C2148H3447N633O681S20
Total number of atoms: 6929

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    33585
Abs 0.1% (=1 g/l)   0.676, assuming all pairs of Cys residues form cystines


Ext. coefficient    33460
Abs 0.1% (=1 g/l)   0.674, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.13
This classifies the protein as unstable.



Aliphatic index: 71.01

Grand average of hydropathicity (GRAVY): -0.545