ProtParam
User-provided sequence:
10 20 30 40 50 60
MGSRSENVGT VTREGSRVEQ DDVLMDDDSD SSEYVDMWID LFLGRKGHEY FCDVDPEYIT
70 80 90 100 110 120
DRFNLMNLQK TVSKFSYVVQ YIVDDLDDSI LENMTHARLE QLESDSRKLY GLIHARYIIT
130 140 150 160 170 180
IKGLQKMYAK YKEADFGRCP RVYCNLQQLL PVGLHDIPGI DCVKLYCPSC EDLYIPKSSR
190 200 210 220 230 240
HSSIDGAYFG TSFPGMFLQA FPDMVPKHPT KRYVPKIFGF ELHKQAQLTR WQELQRLKLV
250
EKLESKDVDL TKSGGFKT
References and
documentation are available.
Number of amino acids: 258
Molecular weight: 29842.04
Theoretical pI: 5.68
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 33
Atomic composition:
Carbon C 1333
Hydrogen H 2068
Nitrogen N 352
Oxygen O 398
Sulfur S 14
Formula: C
1333H
2068N
352O
398S
14
Total number of atoms: 4165
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 32235
Abs 0.1% (=1 g/l) 1.080, assuming all pairs of Cys residues form cystines
Ext. coefficient 31860
Abs 0.1% (=1 g/l) 1.068, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.58
This classifies the protein as unstable.
Aliphatic index: 80.78
Grand average of hydropathicity (GRAVY): -0.455