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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGSRSENVGT VTREGSRVEQ DDVLMDDDSD SSEYVDMWID LFLGRKGHEY FCDVDPEYIT

70 80 90 100 110 120
DRFNLMNLQK TVSKFSYVVQ YIVDDLDDSI LENMTHARLE QLESDSRKLY GLIHARYIIT

130 140 150 160 170 180
IKGLQKMYAK YKEADFGRCP RVYCNLQQLL PVGLHDIPGI DCVKLYCPSC EDLYIPKSSR

190 200 210 220 230 240
HSSIDGAYFG TSFPGMFLQA FPDMVPKHPT KRYVPKIFGF ELHKQAQLTR WQELQRLKLV

250
EKLESKDVDL TKSGGFKT


References and documentation are available.

Number of amino acids: 258 Molecular weight: 29842.04 Theoretical pI: 5.68
Amino acid composition: 
Ala (A) 7 2.7% Arg (R) 14 5.4% Asn (N) 5 1.9% Asp (D) 24 9.3% Cys (C) 6 2.3% Gln (Q) 12 4.7% Glu (E) 15 5.8% Gly (G) 16 6.2% His (H) 7 2.7% Ile (I) 13 5.0% Leu (L) 26 10.1% Lys (K) 19 7.4% Met (M) 8 3.1% Phe (F) 12 4.7% Pro (P) 11 4.3% Ser (S) 19 7.4% Thr (T) 11 4.3% Trp (W) 2 0.8% Tyr (Y) 14 5.4% Val (V) 17 6.6% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 39 Total number of positively charged residues (Arg + Lys): 33 Atomic composition: Carbon C 1333 Hydrogen H 2068 Nitrogen N 352 Oxygen O 398 Sulfur S 14 Formula: C1333H2068N352O398S14 Total number of atoms: 4165 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 32235 Abs 0.1% (=1 g/l) 1.080, assuming all pairs of Cys residues form cystines Ext. coefficient 31860 Abs 0.1% (=1 g/l) 1.068, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 41.58 This classifies the protein as unstable. Aliphatic index: 80.78 Grand average of hydropathicity (GRAVY): -0.455