ProtParam
User-provided sequence:
10 20 30 40 50 60
MGSSASKTAS SVSSASSSSA FSPPPTKFSS DSSSPAVQRA FSFPTPLVHH PPARKGDSHH
70 80 90 100 110 120
LVSLTSTSYG SLLLLDLDGS KNSSSDQQTL PRISISGKNT PDPVSPDSVI NTWELMDGLD
130 140 150 160 170 180
DEFEFEIPKP GKRLNSDFCS KPDPNRNVSL NGSSLKLDES YEIVRIEEDD GDWVPLTYKP
190 200 210 220 230 240
KQPLWKHLSE ESFLSDLDPS IVSSYKKALS SKLLSNHSNT RNPHRPTKSL SCSPSSNPSI
250 260 270 280 290 300
LISEEPKSVS SSQLISSPAK PRLPGTEDKI VLYFTTLRGI RKTYEDCCCV RAILRGVQVS
310 320 330 340 350 360
VDERDISMDS KYRKELQSVL GAAEKPVCLP QVFIRGTHIG GVEEIMQLND GGELAEMLKD
370 380 390 400 410
FPACERLGTC RSCGDARFVP CTNCDGSTKV FEEQDERFKR CPKCNENGLV RCRVCCL
References and
documentation are available.
Number of amino acids: 417
Molecular weight: 45718.47
Theoretical pI: 6.12
Total number of negatively charged residues (Asp + Glu): 54
Total number of positively charged residues (Arg + Lys): 50
Atomic composition:
Carbon C 1979
Hydrogen H 3161
Nitrogen N 557
Oxygen O 643
Sulfur S 21
Formula: C
1979H
3161N
557O
643S
21
Total number of atoms: 6361
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 27930
Abs 0.1% (=1 g/l) 0.611, assuming all pairs of Cys residues form cystines
Ext. coefficient 26930
Abs 0.1% (=1 g/l) 0.589, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 51.02
This classifies the protein as unstable.
Aliphatic index: 72.66
Grand average of hydropathicity (GRAVY): -0.502