ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGSSINYPGF VTKSAHLADT STDASISCEE ATSSQEAKKN FFQRDYNMMK KAPAPTKSKL 

        70         80         90        100        110        120 
SLALQTSKSS SSANGTVQED TSSKTEDFST KSIKKKPDSG VESHVSIQSD SGPQSDSDLD 

       130        140        150        160        170        180 
SDSSISSCDE RNEESLKDYR PGGYHPAFKG EPYKDARYIL VRKLGWGHFS TVWLAKDMVN 

       190        200        210        220        230        240 
NTHVAMKIVR GDKVYTEAAE DEIKLLQRVN DADNTKEDSM GANHILKLLD HFNHKGPNGV 

       250        260        270        280        290        300 
HVVMVFEVLG ENLLALIKKY EHRGIPLIYV KQISKQLLLG LDYMHRRCGI IHTDIKPENV 

       310        320        330        340        350        360 
LMEIGDVEGI VQMVEALDKQ KREAKRLQRH VSRSSDITAN DSSDEKWAEC QTSMPCGSSS 

       370        380        390        400        410        420 
NSKSRSIEKD LSKRCFRRPR RHTIITGSQP LPSPISSSNF FEMRAHFCGS SHNSFSSVSG 

       430        440        450        460        470        480 
NRNIPSSINN NSINNGIGIK NSNNSFLNSV PHSVTRMFIN EDSNDNNNND NSKNKNNNNN 

       490        500        510        520        530        540 
NSNNNNNEDI MNTPLHEEQL ADSLSTFDIS NISQSSDTNG PYISNTMDSN SNVSTDINSP 

       550        560        570        580        590        600 
ENLIQIKIAD LGNACWYDEH YTNSIQTREY RSPEVLLGAP WGCGADIWST ACLIFELITG 

       610        620        630        640        650        660 
DFLFEPDEGH SYTKDDDHIA QIIELLGELP SYLLRNGKYT RTFFNSRGLL RNISKLKFWP 

       670        680        690        700        710        720 
LEDVLTEKYK FSKDEAKEIS DFLSPMLQLD PRKRADAGGL VNHPWLKDTL GMEEIRVPDR 

       730        740 
ELYGSGSDIP GWFEEVRDHK RH 

References and documentation are available.

Number of amino acids: 742

Molecular weight: 83237.50

Theoretical pI: 5.82

Amino acid composition: 
Ala (A)  33	  4.4%
Arg (R)  35	  4.7%
Asn (N)  62	  8.4%
Asp (D)  55	  7.4%
Cys (C)  10	  1.3%
Gln (Q)  20	  2.7%
Glu (E)  48	  6.5%
Gly (G)  42	  5.7%
His (H)  24	  3.2%
Ile (I)  48	  6.5%
Leu (L)  56	  7.5%
Lys (K)  51	  6.9%
Met (M)  17	  2.3%
Phe (F)  25	  3.4%
Pro (P)  31	  4.2%
Ser (S)  92	 12.4%
Thr (T)  34	  4.6%
Trp (W)   9	  1.2%
Tyr (Y)  19	  2.6%
Val (V)  31	  4.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 103
Total number of positively charged residues (Arg + Lys): 86

Atomic composition:

Carbon      C	      3607
Hydrogen    H	      5663
Nitrogen    N	      1037
Oxygen      O	      1176
Sulfur      S	        27

Formula: C3607H5663N1037O1176S27
Total number of atoms: 11510

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    78435
Abs 0.1% (=1 g/l)   0.942, assuming all pairs of Cys residues form cystines


Ext. coefficient    77810
Abs 0.1% (=1 g/l)   0.935, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.66
This classifies the protein as unstable.



Aliphatic index: 71.23

Grand average of hydropathicity (GRAVY): -0.716