ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGSSPAPFAD IGKKAKDLLN KDYIFDHKFT LTMLSATGTE FVATGLKKDD FFFGDISTLY 

        70         80         90        100        110        120 
KGQNTIVDLK IDSHSSVSTK VTLKNLLPSA KAVISFKIPD HKSGKLDVQY VHPHATLNSS 

       130        140        150        160        170        180 
IGLNPTPLLD LSATIGSQNV CLGGEVSFDT ASSSLTKYNA GIGFNNQGVS AALILEDKGE 

       190        200        210        220        230        240 
SLRATYVHTV NPTTSFGAEL IRRFSNYNNS FTVGSSHSVD QFTVVKTRFS NSGKAGMVVQ 

       250        260        270 
REWRPKSHIT FSAEYDSKAV TSSPKLGLAL ALKP 

References and documentation are available.

Number of amino acids: 274

Molecular weight: 29505.43

Theoretical pI: 9.23

Amino acid composition: 
Ala (A)  20	  7.3%
Arg (R)   6	  2.2%
Asn (N)  14	  5.1%
Asp (D)  16	  5.8%
Cys (C)   1	  0.4%
Gln (Q)   6	  2.2%
Glu (E)   7	  2.6%
Gly (G)  20	  7.3%
His (H)   8	  2.9%
Ile (I)  13	  4.7%
Leu (L)  26	  9.5%
Lys (K)  23	  8.4%
Met (M)   3	  1.1%
Phe (F)  16	  5.8%
Pro (P)  11	  4.0%
Ser (S)  34	 12.4%
Thr (T)  23	  8.4%
Trp (W)   1	  0.4%
Tyr (Y)   7	  2.6%
Val (V)  19	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 29

Atomic composition:

Carbon      C	      1321
Hydrogen    H	      2084
Nitrogen    N	       352
Oxygen      O	       405
Sulfur      S	         4

Formula: C1321H2084N352O405S4
Total number of atoms: 4166

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    15930
Abs 0.1% (=1 g/l)   0.540, assuming all pairs of Cys residues form cystines


Ext. coefficient    15930
Abs 0.1% (=1 g/l)   0.540, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 23.79
This classifies the protein as stable.



Aliphatic index: 82.92

Grand average of hydropathicity (GRAVY): -0.166