ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGSVGKLAYI IYLHKGPRIN GLRRDPESYL RNPSGVLFTE VNAKECQDKV RSILQLPKYG 

        70         80         90        100        110        120 
INLSNELILQ CLTHKSFAHG SKPYNEKLNL LGAQFLKLQT CIHSLKNGSP AESCENGQLS 

       130        140        150        160        170        180 
LQFSNLGTKF AKELTSKNTA CTFVKLHNLD PFIFWKMRDP IKDGHINGET TIFASVLNAF 

       190        200        210        220        230 
IGAILSTNGS EKAAKFIQGS LLDKEDLHSL VNIANENVAS AKAKISDKEN KAFL 

References and documentation are available.

Number of amino acids: 234

Molecular weight: 25850.76

Theoretical pI: 9.21

Amino acid composition: 
Ala (A)  17	  7.3%
Arg (R)   6	  2.6%
Asn (N)  19	  8.1%
Asp (D)   8	  3.4%
Cys (C)   5	  2.1%
Gln (Q)   8	  3.4%
Glu (E)  13	  5.6%
Gly (G)  16	  6.8%
His (H)   7	  3.0%
Ile (I)  16	  6.8%
Leu (L)  30	 12.8%
Lys (K)  23	  9.8%
Met (M)   2	  0.9%
Phe (F)  12	  5.1%
Pro (P)   8	  3.4%
Ser (S)  20	  8.5%
Thr (T)  10	  4.3%
Trp (W)   1	  0.4%
Tyr (Y)   5	  2.1%
Val (V)   8	  3.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 21
Total number of positively charged residues (Arg + Lys): 29

Atomic composition:

Carbon      C	      1157
Hydrogen    H	      1851
Nitrogen    N	       317
Oxygen      O	       339
Sulfur      S	         7

Formula: C1157H1851N317O339S7
Total number of atoms: 3671

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    13200
Abs 0.1% (=1 g/l)   0.511, assuming all pairs of Cys residues form cystines


Ext. coefficient    12950
Abs 0.1% (=1 g/l)   0.501, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.86
This classifies the protein as stable.



Aliphatic index: 93.85

Grand average of hydropathicity (GRAVY): -0.242