ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGSVNSSPNE EFETVPDSQI SGFDSPLIPT SVGSYFRDDD DDEKVHPNFI SDPENDSLNS 

        70         80         90        100        110        120 
DEEFSSLENS DLNLSGAKAE SGDDFDPILK RTIISKRKAP SNNEDEEIVK TPRKLVNYVP 

       130        140        150        160        170        180 
LKIFNLGDSF DDTITTTVAK LQDLKKEILD SPRSNKSIVI TSNTVAKSEL QKSIKFSGSI 

       190        200        210        220        230        240 
PEIYLDVVTK ETISDKYKDW HFISKNCHYE QLMDLEMKDT AYSFLFGSSR SQGKVPEFVH 

       250        260        270        280        290        300 
LKCPSITNLL VLFGVNQEKC NSLKINYEKK ENSRYDNLCT IFPVNKMLKF LMYFYSDDDN 

       310        320        330        340        350        360 
DDVREFFLKA FICLILDRKV FNAMESDHRL CFKVLELFNE AHFINSYFEI VDKNDFFLHY 

       370        380        390        400        410        420 
RLLQIFPHLQ SALLRRRFSE KQGRTETIQQ NIIKEFNEFF DCKNYKNLLY FILTMYGSKF 

       430        440        450        460 
IPFGPKCQVT EYFKDCILDI SNETTNDVEI SILKGILNLF SKIR 

References and documentation are available.

Number of amino acids: 464

Molecular weight: 53771.96

Theoretical pI: 5.12

Amino acid composition: 
Ala (A)  10	  2.2%
Arg (R)  16	  3.4%
Asn (N)  33	  7.1%
Asp (D)  39	  8.4%
Cys (C)   9	  1.9%
Gln (Q)  12	  2.6%
Glu (E)  34	  7.3%
Gly (G)  14	  3.0%
His (H)   8	  1.7%
Ile (I)  36	  7.8%
Leu (L)  44	  9.5%
Lys (K)  41	  8.8%
Met (M)   7	  1.5%
Phe (F)  37	  8.0%
Pro (P)  18	  3.9%
Ser (S)  45	  9.7%
Thr (T)  21	  4.5%
Trp (W)   1	  0.2%
Tyr (Y)  16	  3.4%
Val (V)  23	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 73
Total number of positively charged residues (Arg + Lys): 57

Atomic composition:

Carbon      C	      2420
Hydrogen    H	      3741
Nitrogen    N	       615
Oxygen      O	       738
Sulfur      S	        16

Formula: C2420H3741N615O738S16
Total number of atoms: 7530

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    29840
Abs 0.1% (=1 g/l)   0.555, assuming all pairs of Cys residues form cystines


Ext. coefficient    29340
Abs 0.1% (=1 g/l)   0.546, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.84
This classifies the protein as unstable.



Aliphatic index: 83.77

Grand average of hydropathicity (GRAVY): -0.418