ProtParam
User-provided sequence:
10 20 30 40 50 60
MGTGKKEKSR RIREGDTKDG NLRVKGENFY RDSKRVKFLN MYTSGKEIRN KKGNLIRAAS
70 80 90 100 110 120
FQDSTIPDAR VQPDRRWFGN TRVISQDALQ HFRSALGETQ KDTYQVLLRR NKLPMSLLEE
130 140 150 160 170 180
KDADESPKAR ILDTESYADA FGPKAQRKRP RLAASNLEDL VKATNEDITK YEEKQVLDAT
190 200 210 220 230 240
LGLMGNQEDK ENGWTSAAKE AIFSKGQSKR IWNELYKVID SSDVVIHVLD ARDPLGTRCK
250 260 270 280 290 300
SVEEYMKKET PHKHLIYVLN KCDLVPTWVA AAWVKHLSKE RPTLAFHASI TNSFGKGSLI
310 320 330 340 350 360
QLLRQFSQLH TDRKQISVGF IGYPNTGKSS IINTLRKKKV CQVAPIPGET KVWQYITLMK
370 380 390 400 410 420
RIFLIDCPGI VPPSSKDSEE DILFRGVVRV EHVTHPEQYI PGVLKRCQVK HLERTYEISG
430 440 450 460 470 480
WKDATEFIEI LARKQGRLLK GGEPDESGVS KQILNDFNRG KIPWFVLPPE KEGEEKPKKK
EVEKTA
References and
documentation are available.
Number of amino acids: 486
Molecular weight: 55488.59
Theoretical pI: 9.52
Total number of negatively charged residues (Asp + Glu): 66
Total number of positively charged residues (Arg + Lys): 87
Atomic composition:
Carbon C 2466
Hydrogen H 3967
Nitrogen N 707
Oxygen O 726
Sulfur S 11
Formula: C
2466H
3967N
707O
726S
11
Total number of atoms: 7877
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 62130
Abs 0.1% (=1 g/l) 1.120, assuming all pairs of Cys residues form cystines
Ext. coefficient 61880
Abs 0.1% (=1 g/l) 1.115, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.94
This classifies the protein as stable.
Aliphatic index: 79.63
Grand average of hydropathicity (GRAVY): -0.700