ProtParam
User-provided sequence:
10 20 30 40 50 60
MGTHVAPWKQ LLFGAIEANS HLSHSSYVQL ATIGLNGRPS NRTVVFRGFE ENSDRIQINT
70 80 90 100 110 120
DLRSRKIEEL KHCPFSEMCW YFSDTWEQFR INGRIEVIDA SNPDQTKLQQ REKAWFANSL
130 140 150 160 170 180
RSRLIYVCPT PGSPCNSEQS SQQVKLDPSS GPVPEYCLLL LEPEKVDYLN LKTNQRLFFS
190
SMATGTGEKC WTSEKVNP
References and
documentation are available.
Number of amino acids: 198
Molecular weight: 22619.53
Theoretical pI: 6.89
Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 22
Atomic composition:
Carbon C 999
Hydrogen H 1550
Nitrogen N 280
Oxygen O 303
Sulfur S 9
Formula: C
999H
1550N
280O
303S
9
Total number of atoms: 3141
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 35325
Abs 0.1% (=1 g/l) 1.562, assuming all pairs of Cys residues form cystines
Ext. coefficient 34950
Abs 0.1% (=1 g/l) 1.545, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.09
This classifies the protein as unstable.
Aliphatic index: 71.87
Grand average of hydropathicity (GRAVY): -0.560