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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGTHVAPWKQ LLFGAIEANS HLSHSSYVQL ATIGLNGRPS NRTVVFRGFE ENSDRIQINT

70 80 90 100 110 120
DLRSRKIEEL KHCPFSEMCW YFSDTWEQFR INGRIEVIDA SNPDQTKLQQ REKAWFANSL

130 140 150 160 170 180
RSRLIYVCPT PGSPCNSEQS SQQVKLDPSS GPVPEYCLLL LEPEKVDYLN LKTNQRLFFS

190
SMATGTGEKC WTSEKVNP


References and documentation are available.

Number of amino acids: 198 Molecular weight: 22619.53 Theoretical pI: 6.89
Amino acid composition: 
Ala (A) 8 4.0% Arg (R) 12 6.1% Asn (N) 12 6.1% Asp (D) 7 3.5% Cys (C) 6 3.0% Gln (Q) 11 5.6% Glu (E) 15 7.6% Gly (G) 10 5.1% His (H) 4 2.0% Ile (I) 9 4.5% Leu (L) 18 9.1% Lys (K) 10 5.1% Met (M) 3 1.5% Phe (F) 9 4.5% Pro (P) 12 6.1% Ser (S) 21 10.6% Thr (T) 11 5.6% Trp (W) 5 2.5% Tyr (Y) 5 2.5% Val (V) 10 5.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 22 Total number of positively charged residues (Arg + Lys): 22 Atomic composition: Carbon C 999 Hydrogen H 1550 Nitrogen N 280 Oxygen O 303 Sulfur S 9 Formula: C999H1550N280O303S9 Total number of atoms: 3141 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 35325 Abs 0.1% (=1 g/l) 1.562, assuming all pairs of Cys residues form cystines Ext. coefficient 34950 Abs 0.1% (=1 g/l) 1.545, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 48.09 This classifies the protein as unstable. Aliphatic index: 71.87 Grand average of hydropathicity (GRAVY): -0.560