ProtParam
User-provided sequence:
10 20 30 40 50 60
MGTITVVINE GPILLIRALH RATTNKKMFR STVWRRFAST GEIAKAKLDE FLIYHKTDAK
70 80 90 100 110 120
LKPFIYRPKN AQILLTKDIR DPKTREPLQP RPPVKPLSKQ TLNDFIYSVE PNSTELLDWF
130 140 150 160 170 180
KEWTGTSIRK RAIWTYISPI HVQKMLTASF FKIGKYAHMV GLLYGIEHKF LKAQNPSVFD
190 200 210 220 230 240
IEHFFNTNIM CALHRNRLKD YKDAEIAQRK LQVAWKKVLN RKNNTGLANI LVATLGRQIG
250 260 270 280 290 300
FTPELTGLQP VDISLPDIPN SSSGAELKDL LSKYEGIYLI ARTLLDIDQH NAQYLELQEF
310 320 330
IRQYQNALSE SSDPYDTHLK ALGLLETPPP QESTEKEEK
References and
documentation are available.
Number of amino acids: 339
Molecular weight: 38988.09
Theoretical pI: 9.45
Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 48
Atomic composition:
Carbon C 1773
Hydrogen H 2813
Nitrogen N 477
Oxygen O 499
Sulfur S 6
Formula: C
1773H
2813N
477O
499S
6
Total number of atoms: 5568
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 45380
Abs 0.1% (=1 g/l) 1.164, assuming all pairs of Cys residues form cystines
Ext. coefficient 45380
Abs 0.1% (=1 g/l) 1.164, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.19
This classifies the protein as stable.
Aliphatic index: 93.83
Grand average of hydropathicity (GRAVY): -0.410