ProtParam
User-provided sequence:
10 20 30 40 50 60
MGTLFRRNVQ NQKSDSDENN KGGSVHNKRE SRNHIHHQQG LGHKRRRGIS GSAKRNERGK
70 80 90 100 110 120
DFDRKRDGNG RKRWRDSRRL IFILGAFLGV LLPFSFGAYH VHNSDSDLFD NFVNFDSLKV
130 140 150 160 170 180
YLDDWKDVLP QGISSFIDDI QAGNYSTSSL DDLSENFAVG KQLLRDYNIE AKHPVVMVPG
190 200 210 220 230 240
VISTGIESWG VIGDDECDSS AHFRKRLWGS FYMLRTMVMD KVCWLKHVML DPETGLDPPN
250 260 270 280 290 300
FTLRAAQGFE STDYFIAGYW IWNKVFQNLG VIGYEPNKMT SAAYDWRLAY LDLERRDRYF
310 320 330 340 350 360
TKLKEQIELF HQLSGEKVCL IGHSMGSQII FYFMKWVEAE GPLYGNGGRG WVNEHIDSFI
370 380 390 400 410 420
NAAGTLLGAP KAVPALISGE MKDTIQLNTL AMYGLEKFFS RIERVKMLQT WGGIPSMLPK
430 440 450 460 470 480
GEEVIWGDMK SSSEDALNNN TDTYGNFIRF ERNTSDAFNK NLTMKDAINM TLSISPEWLQ
490 500 510 520 530 540
RRVHEQYSFG YSKNEEELRK NELHHKHWSN PMEVPLPEAP HMKIYCIYGV NNPTERAYVY
550 560 570 580 590 600
KEEDDSSALN LTIDYESKQP VFLTEGDGTV PLVAHSMCHK WAQGASPYNP AGINVTIVEM
610 620 630 640 650 660
KHQPDRFDIR GGAKSAEHVD ILGSAELNDY ILKIASGNGD LVEPRQLSNL SQWVSQMPFP
M
References and
documentation are available.
Number of amino acids: 661
Molecular weight: 75393.13
Theoretical pI: 6.21
Total number of negatively charged residues (Asp + Glu): 85
Total number of positively charged residues (Arg + Lys): 76
Atomic composition:
Carbon C 3361
Hydrogen H 5148
Nitrogen N 934
Oxygen O 993
Sulfur S 27
Formula: C
3361H
5148N
934O
993S
27
Total number of atoms: 10463
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 124010
Abs 0.1% (=1 g/l) 1.645, assuming all pairs of Cys residues form cystines
Ext. coefficient 123760
Abs 0.1% (=1 g/l) 1.642, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.28
This classifies the protein as unstable.
Aliphatic index: 75.22
Grand average of hydropathicity (GRAVY): -0.522