ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGTTTSHPAQ KKQTTKKCRA PIMSDVREKP SNAQGCEPQE MDAVSKKVTE LSLNKCSDSQ 

        70         80         90        100        110        120 
DAGQPSREGS ITKKKSTLLL RDEDEPTMPK LSVMETAVDT DSGSSSTSDD EEGDIIAQTT 

       130        140        150        160        170        180 
EPKQDASPDD DRSGHSSPRE EGQQQIRAKE ASGGPSEIKS SLMVPVEIRW QQGGSKVYVT 

       190        200        210        220        230        240 
GSFTKWRKMI GLIPDSDNNG SFHVKLRLLP GTHRFRFIVD NELRVSDFLP TATDQMGNFV 

       250        260        270        280        290        300 
NYIEVRQPEK NPTNEKIRSK EADSMRPPTS DRSSIALQIG KDPDDFGDGY TRFHEDLSPR 

       310        320        330        340        350        360 
PPLEYTTDIP AVFTDPSVME RYYYTLDRQQ SNTDTSWLTP PQLPPQLENV ILNKYYATQD 

       370        380        390        400        410 
QFNENNSGAL PIPNHVVLNH LVTSSIKHNT LCVASIVRYK QKYVTQILYT PIESS 

References and documentation are available.

Number of amino acids: 415

Molecular weight: 46404.65

Theoretical pI: 5.44

Amino acid composition: 
Ala (A)  17	  4.1%
Arg (R)  22	  5.3%
Asn (N)  18	  4.3%
Asp (D)  32	  7.7%
Cys (C)   4	  1.0%
Gln (Q)  24	  5.8%
Glu (E)  27	  6.5%
Gly (G)  21	  5.1%
His (H)   8	  1.9%
Ile (I)  21	  5.1%
Leu (L)  26	  6.3%
Lys (K)  27	  6.5%
Met (M)  10	  2.4%
Phe (F)  10	  2.4%
Pro (P)  32	  7.7%
Ser (S)  44	 10.6%
Thr (T)  34	  8.2%
Trp (W)   3	  0.7%
Tyr (Y)  12	  2.9%
Val (V)  23	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 49

Atomic composition:

Carbon      C	      2008
Hydrogen    H	      3187
Nitrogen    N	       569
Oxygen      O	       666
Sulfur      S	        14

Formula: C2008H3187N569O666S14
Total number of atoms: 6444

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    34630
Abs 0.1% (=1 g/l)   0.746, assuming all pairs of Cys residues form cystines


Ext. coefficient    34380
Abs 0.1% (=1 g/l)   0.741, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.65
This classifies the protein as unstable.



Aliphatic index: 64.34

Grand average of hydropathicity (GRAVY): -0.826