ProtParam
User-provided sequence:
10 20 30 40 50 60
MGVADLIKKF ESISKEEGDA TVDTNSSSKP LKSNDETKEL HQQESTAVPQ EVDVNEEFEN
70 80 90 100 110 120
EPETINSSRT AEKPLETNLP KPETNEEDEE EGSMSENKIY SKGENADINV NDFQEYKEME
130 140 150 160 170 180
NTGAEVLASS VEESDAIQEG VAEETEGIAT PKQKENEKND ESEEESANNA SEPAEEYSQS
190 200 210 220 230 240
EEDADIEQSN GKETENAENA SQQANDGSTS TTTSKNKKKK NKKKNKKKRN GNVNTNANVD
250 260 270 280 290 300
DSTKTGENDD TTGDTTSSTT SAIQEVNDLE VVDDSCLGID QQHNREHLKA LTQDVKEETL
310 320 330 340 350 360
ENIAHEGRGD NTGDQNAVEK SDFEKSDTEG SRIGRDLPFE FGKRNLTEES DVWDHNAWDN
370 380 390 400 410 420
VEWGEEQVQQ AEEKIKEQFK HPVPEFDKKL YNENPARYWD IFYKNNKENF FKDRKWLQIE
430 440 450 460 470 480
FPILYASTRK DAEPVTIFEI GCGAGNTFFP ILKDNENENL RIIAADFAPR AVELVKNSEQ
490 500 510 520 530 540
FNPKYGHATV WDLANPDGNL PDGVEPHSVD IAVMIFVFSA LAPNQWDQAM DNLHKILKPG
550 560 570 580 590 600
GKIIFRDYGA YDLTQVRFKK NRILEENFYV RGDGTRVYFF SEEKLREIFT KKYFLENKIG
610 620
TDRRLLVNRK RQLKMYRCWV QAVFDVPQ
References and
documentation are available.
Number of amino acids: 628
Molecular weight: 71485.90
Theoretical pI: 4.62
Total number of negatively charged residues (Asp + Glu): 132
Total number of positively charged residues (Arg + Lys): 79
Atomic composition:
Carbon C 3098
Hydrogen H 4804
Nitrogen N 862
Oxygen O 1067
Sulfur S 9
Formula: C
3098H
4804N
862O
1067S
9
Total number of atoms: 9840
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 64985
Abs 0.1% (=1 g/l) 0.909, assuming all pairs of Cys residues form cystines
Ext. coefficient 64860
Abs 0.1% (=1 g/l) 0.907, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.86
This classifies the protein as unstable.
Aliphatic index: 59.79
Grand average of hydropathicity (GRAVY): -1.074