ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGVIKKKRSH HGKASRQQYY SGVQVGGVGS MGAINNNIPS LTSFAEENNY QYGYSGSSAG 

        70         80         90        100        110        120 
MNGRSLTYAQ QQLNKQRQDF ERVRLRPEQL SNIIHDESDT ISFRSNLLKN FISSNDAFNM 

       130        140        150        160        170        180 
LSLTTVPCDR IEKSRLFSEK TIRYLMQKQH EMKTQAAELQ EKPLTPLKYT KLIAAAEDGS 

       190        200        210        220        230        240 
RSTKDMIDAV FEQDSHLRYQ PDGVVVHRDD PALVGKLRGD LREAPADYWT HAYRDVLAQY 

       250        260        270        280        290        300 
HEAKERIRQK EVTAGEAQDE ASLQQQQQQD LQQQQQVVTT VASQSPHATA TEKEPVPAVV 

       310        320        330 
DDPLENMFGD YSNEPFNTNF DDEFGDLDAV FF 

References and documentation are available.

Number of amino acids: 332

Molecular weight: 37606.66

Theoretical pI: 5.44

Amino acid composition: 
Ala (A)  26	  7.8%
Arg (R)  19	  5.7%
Asn (N)  16	  4.8%
Asp (D)  24	  7.2%
Cys (C)   1	  0.3%
Gln (Q)  31	  9.3%
Glu (E)  23	  6.9%
Gly (G)  18	  5.4%
His (H)   9	  2.7%
Ile (I)  12	  3.6%
Leu (L)  24	  7.2%
Lys (K)  18	  5.4%
Met (M)   8	  2.4%
Phe (F)  13	  3.9%
Pro (P)  13	  3.9%
Ser (S)  26	  7.8%
Thr (T)  17	  5.1%
Trp (W)   1	  0.3%
Tyr (Y)  13	  3.9%
Val (V)  20	  6.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	      1635
Hydrogen    H	      2555
Nitrogen    N	       473
Oxygen      O	       530
Sulfur      S	         9

Formula: C1635H2555N473O530S9
Total number of atoms: 5202

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24870
Abs 0.1% (=1 g/l)   0.661, assuming all pairs of Cys residues form cystines


Ext. coefficient    24870
Abs 0.1% (=1 g/l)   0.661, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.96
This classifies the protein as unstable.



Aliphatic index: 67.59

Grand average of hydropathicity (GRAVY): -0.789